Migration and 3D Traction Force Measurements inside Compliant Microchannels

Date

2022-09-01

Author

Afthinos, Alexandros
Bera, Kaustav
Chen, Junjie
Özçelikkale, Altuğ
Amitrano, Alice
Choudhury, Mohammad Ikbal
Huang, Randy
Pachidis, Pavlos
Mistriotis, Panagiotis
Chen, Yun
Konstantopoulos, Konstantinos

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Cells migrate in vivo through channel-like tracks. While polydimethylsiloxane devices emulate such tracks in vitro, their channel walls are impermeable and have supraphysiological stiffness. Existing hydrogel-based platforms address these issues but cannot provide high-throughput analysis of cell motility in independently controllable stiffness and confinement. We herein develop polyacrylamide (PA)-based microchannels of physiological stiffness and prescribed dimensions for high-throughput analysis of cell migration and identify a biphasic dependence of speed upon confinement and stiffness. By utilizing novel four-walled microchannels with heterogeneous stiffness, we reveal the distinct contributions of apicolateral versus basal microchannel wall stiffness to confined versus unconfined migration. While the basal wall stiffness dictates unconfined migration, apicolateral stiffness controls confined migration. By tracking nanobeads embedded within channel walls, we innovate three-dimensional traction force measurements around spatially confining cells at subcellular resolution. Our unique and highly customizable device fabrication strategy provides a physiologically relevant in vitro platform to study confined cells.

Subject Keywords

cell migration, confinement, traction force microscopy, soft substrate, microfluidics, MATRIX STIFFNESS, CELL-MIGRATION, CONFINEMENT, MICROENVIRONMENT, HYDROGELS, MOTILITY, INVASION, BREAST

URI

https://hdl.handle.net/11511/100823

Journal

NANO LETTERS

DOI

https://doi.org/10.1021/acs.nanolett.2c01261

Collections

Department of Mechanical Engineering, Article

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Citation Formats

A. Afthinos et al., “Migration and 3D Traction Force Measurements inside Compliant Microchannels,” NANO LETTERS, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/100823.