Enteric Glia Response Upon Intestinal Damage

2022-10
Sarı, İlke
Demirdizen, Mert
Değirmenci, Bahar
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The enteric nervous system consists of distinct types of neurons and glia is a part of the autonomic nervous system that regulates the homeostasis of the gastrointestinal tract [1,2]. However, mechanisms of enteric glia in inflammation and recovery in colon remain unresolved. Here, we show that S100b+ enteric glia and Pdgfra+ mesenchymal cells cooperate in inflammation state. Immunohistochemistry analysis of inflammatory colon reveals S100b+ enteric glia and Pdgfra+ mesenchymal cell migration from myenteric plexus to mucosa. To understand changed cellular composition of the colon depending on damage, we used spatial transcriptomics data obtained from DSS treated recovering mouse [3]. Stromal scRNAseq data from three round DSS treated mouse [4] used to deconvolute spots with the use of cell2location [5]. At mild damage, edema, and recovered regions, different cellular compositions were observed. Abundances and locations of different fibroblast types and glial cell types were different with changed morphology of the colon. To find out changed expression of the cell types which populated at damaged region, receptor-ligand analysis was done to find out changed expressions of DSS treated and control stromal scRNAseq data with use of scDiffCom [6]. The gene ontology analysis of the most significantly changed expressions were related to wound healing, toxic substance response, and immune responses. Some genes were selected to understand whether, depending on the damage, the expressions mimic embryonic development in humans. 12 PWC, 19 PWC, and healthy human spatial transcriptomics data used [7]. To deconvolute fetal fibroblast and neural scRNAseq data [7] for 12 and 19 PWC slides and healthy stromal human scRNAseq data [8] for adult slide were used. Our findings indicate that some responses to damage imitates embryonic development. This study has been funded by EMBO IG and TUBITAK 1001 (Grant No. 219Z163).
https://hibit2022.ims.metu.edu.tr
https://hdl.handle.net/11511/101352
Graduate School of Informatics, Conference / Seminar

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Citation Formats
İ. Sarı, M. Demirdizen, and B. Değirmenci, “Enteric Glia Response Upon Intestinal Damage,” Erdemli, Mersin, TÜRKİYE, 2022, p. 3112, Accessed: 00, 2023. [Online]. Available: https://hibit2022.ims.metu.edu.tr.
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