Docking of alphafold structures of human taste receptors to glutamate and aspartame identifies novel binding residues

Kuzucu, Havva Nurefşan
Karaman, Merih
Türköz, Burcu Kaplan
L-glutamic acid salt and aspartame are commonly used taste enhancer food additives. L-glutamic acid and aspartame bind to Venus flytrap domain (VFT) of T1R1 and T1R2 respectively to form umami and sweet taste. In this research, aspartame and L-glutamic acid binding sites on human T1R1 (hT1R1) and T1R2 (hT1R2) were analyzed via docking. Both structures were downloaded from AlphaFold Database, uploaded to HADDOCK 2.4 docking server with their corresponding ligands and results were further analyzed via Verify-3D server. After docking simulation of hT1R1 and glutamate, similar binding characteristics of glutamate to Metabotropic Glutamate Receptor was obtained. Docking results of hT1R2 and aspartame resulted in identification of overall five novel residues on hT1R2 as compared to previous studies. Overall, our analysis provided novel binding sites on both the taste receptors. The results will be valuable in understanding taste receptors mechanism of action.


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Citation Formats
H. N. Kuzucu, M. Karaman, and B. K. Türköz, “Docking of alphafold structures of human taste receptors to glutamate and aspartame identifies novel binding residues,” Erdemli, Mersin, TÜRKİYE, 2022, p. 3059, Accessed: 00, 2023. [Online]. Available: