Enzyme-assisted extraction of antioxidants from banana peel

Date

2023-7-25

Author

Katırcı, Sinem

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Fruit and vegetable processing wastes contain bioactive compounds such as polyphenols. In this study, polyphenolics were extracted from dried local Anamur banana peels using enzyme-assisted extraction (EAE). Ultrasound-assisted extraction (UAE) with aqueous ethanol and EAE-UAE combination were also applied for comparison. Pectinex ultra SP-L and celluclast 1.5 L in sodium acetate buffer (pH 5) were used for EAE. According to the Box-Behnken design optimization results, the highest total phenolic content (TPC) was obtained by EAE with 1% pectinex ultra SP-L and 0.736% celluclast 1.5 L for 1 h extraction at 50°C with a solid solvent ratio of 1/10 (w/v). The extraction effectiveness was found as EAE (with buffer: 1.746±0.078 mg GAE/mL extract; with water: 1.633±0.014 mg GAE/mL extract) > EAE-UAE combination (1.537±0.020 mg GAE/mL extract) > UAE with 50% aqueous ethanol (0.759±0.008 mg GAE/mL extract). For antioxidant capacity (AC) estimations, FRAP, CUPRAC, DPPH• and ABTS•+ assays were performed. No significant differences were detected among FRAP and CUPRAC ACs of extracts obtained by EAE with buffer or water and EAE-UAE. According to DPPH• assay maximum scavenging capacity (SCmax), the ACs of EAE with buffer and water were found as same, but UAE with 50% aqueous ethanol and EAE-UAE provided higher ACs. DPPH• half maximal effective antioxidant concentration (EC50) values showed that EAE with buffer or water and EAE-UAE combination gave similar EC50 values. According to ABTS•+ SCmax values, no significant differences were observed ACs for EAE with buffer, EAE with water and EAE-UAE. When ABTS•+ EC50 values were compared, similar effectiveness was obtained for EAE with buffer and EAE-UAE. CUPRAC and ABTS•+ assay can be used for comparison of ACs.

Subject Keywords

Banana Peel, Antioxidant Capacity, Enzyme-assisted Extraction, Ultrasound-assisted Extraction

URI

https://hdl.handle.net/11511/104739

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Graduate School of Natural and Applied Sciences, Thesis