Microbial oil extraction from Lipomyces starkeyi cells by using high pressure

Date

2023-7-24

Author

Batıgün, Ayşe Alara

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

392
views

278
downloads

Cite This

Lipids from microbial sources are used for various purposes, such as enrichment of edible oils and component of dietary supplements. Among the oleaginous yeasts, Lipomyces starkeyi stands out for its ability to accumulate high lipid fractions within its cells. In this study, L. starkeyi biomass was produced in a hydrolyzed cheese whey medium, wherein the lactose in whey was hydrolyzed using β-galactosidase. The lipid was extracted by assistance of novel disruption techniques namely high hydrostatic pressure (HHP) and high-pressure homogenization (HPH) methods supported by the Bligh and Dyer method as well as conventional high-speed homogenizer as a control method. To optimize downstream processing and reduce costs associated with obtaining yeast lipids, wet and dry routes of disruption were evaluated. The HPH method implemented using synthetic media-based biomass via wet route scheme (49.0±0.35%) provided higher lipid yield than dry route HPH method (40.0±3.71%). Similarly, wet route HPH (42.1±0.52%) assisted extraction provided higher lipid yield from whey-based biomass than both wet (24.5±12.6%) and dry (31.9±2.55%) route HHP assisted extraction methods. Additionally, based on SEM analysis, HPH method caused more cellular damage than HHP method. According to FAME analysis, L. starkeyi lipids were rich in palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (C18:1). Thus, L. starkeyi oil proved to be a valuable source of omega-3 and omega-7 fatty acids compared to many plant oils. Furthermore, the lipids produced by L. starkeyi have the potential to substitute palm oil and cocoa butter.

Subject Keywords

Lipomyces starkeyi, Enzymatic lactose hydrolysis, Cheese whey, High-pressure homogenizer, High hydrostatic pressure

URI

https://hdl.handle.net/11511/104829

Collections

Graduate School of Natural and Applied Sciences, Thesis