Rapid prototyping of microfluidic tissue culture systems via mask based photo polymerization

Date

2023-7-12

Author

Dedekargınoğlu, Barış

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Emerging technologies in microfabrication and microfluidics have enabled in vitro models of human physiology that can mimic certain features of native tissue microenvironment in various health and disease conditions. In particular, microfluidic tumor models show great promise for predictive screening of cancer nanomedicine and evaluation of personalized treatment scenarios. However, reliance on standard manufacturing methods limit the ability of such platforms to be rapidly prototyped and tailored according to personal needs. In order to address this problem, this thesis focuses on utilizing consumer grade 3D printers to establish a microfluidic device manufacturing workflow and demonstrate device capabilities by analyzing macromolecule transport as well as mono and coculture of cells on these devices. Results show that while printers can manufacture cavities with good compliance of design values in xy-plane and z-axis, there is a percent loss associated with printed protrusion heights. Macromolecule transport results suggest that successful analysis can be performed, however, unwanted advection effects can become present on devices depending on perfusion strategies. Finally, cell culture experiments show that on device culture is possible for multiple days with the devices.

Subject Keywords

Microfluidics, Microfabrication, 3D printing

URI

https://hdl.handle.net/11511/104861

Collections

Graduate School of Natural and Applied Sciences, Thesis