Investigation of enrichment of denitrifying anaerobic methane oxidation (DAMO) microorganisms and the effect of magnetic nanoparticles on their activity

Date

2023-9

Author

Tolan, Simgenur

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Denitrifying anaerobic methane oxidation (DAMO) is a novel, recently discovered bioprocess. It can overcome anaerobic digester challenges such as loss of dissolved methane via supernatant and lack of nitrogen removal in a complementary way. However, DAMO microorganisms are slowly growing microorganisms. Thus, the topic of shortening the enrichment period or increasing the DAMO activity is extensively studied. In this study, it was aimed to investigate the enrichment of DAMO microorganisms in sequencing batch reactors (SBRs) and determine effects of some parameters (different nitrogen sources and concentrations, HRT, COD, magnetic nanoparticles) on DAMO-Anammox co-culture. One SBR fed with only nitrate was operated to improve mainly DAMO archaea. A second SBR was conducted to mimic anaerobic digester effluent characteristics based on Nconcentrations and COD. Batch studies (SET-1 and SET-2) were performed with Fe2NiO4 and Fe4NiO4Zn Magnetic Nanoparticles (MNP) to investigate the effect of NPs on DAMO activity and DAMO-Anammox co-culture. NO3-as a sole nitrogen source was not observed to be a good choice for DAMO microorganisms. NH4+-N was primarily removed by Anammox bacteria, regardless of COD. On the other hand, DAMO microorganisms were adversely affected by COD. The results of MNP studies revealed that NH4+-N and NO2--N removal efficiency increased from 72% to 88% and from 45% to 56% at 120 µM Fe2NiO4 reactors, respectively, and NO3--N removal efficiency increased from 25% to 36% at 50 µM Fe2NiO4 reactors. Addition of 50 µM Fe2NiO4 decreased lag time of Anammox and DAMO bacteria. Fe4NiO4Zn negatively affected Anammox and DAMO microorganisms.

Subject Keywords

DAMO, Anammox, Magnetic Nanoparticle, Methane, Nitrogen

URI

https://hdl.handle.net/11511/105250

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Graduate School of Natural and Applied Sciences, Thesis