Synthesis And Applications of CBD Loaded Peptide Hydrogels And Biphenylalanine Derivatives

Date

2023-8-24

Author

Baytekin, Batuhan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

106
views

5
downloads

Cite This

This thesis includes two different studies on peptide-based therapeutics. In the first chapter, peptide-based hydrogels were used as a delivery platform for Cannabidiol (CBD). Five different types of antimicrobial peptides were synthesized, and CBD was attempted to be incorporated into individual peptide fibres. Ac- IKFQFHFD-NH2 (PEP2) and Nap-FFKK-OH (PEP5) were found to have low gelation concentration, 2.5 wt% and 0.5 wt% respectively. Their fibers were examined through TEM and SEM. It was found that the two peptides have the encapsulation efficiency of 30-35%. The release studies showed that PEP2 could release up to 70% of CBD, PEP5 up to 20%. In rheological analysis, PEP2 found to have three regeneration cycle unlike PEP5’s one cycle. Cytotoxicity assay of the peptide fibers on A375 cells showed PEP2-CBD increased CBD’s cell viability (101.3%) and showed higher number of migration cells. Peptide fibers also experimented in RT-PCR, anti-inflammatory and antibacterial assay. The potential significance of this research is substantial, and CBD-peptide hydrogels in the project shows promising results. In the second chapter, PSMA-617 derivatives were expected to have higher affinity against pure PSMA protein. Naphthalene group of PSMA-617 coded ligand was changed with different biphenylalanine derivatives (4-H, 4-CN, 4-NO2, 3-AcNH, 4-OH, 4-OMe, 4-AcNH). Biphenylalanine derivatives were characterized via FTIR, HRMS, 1H, and 13C NMR. Then, they were used in PSMA-617 synthesis to evaluate inhibition values. At 0.5 nm, PSMA-617 derivatives had inhibition values of BIP (20%), 4-CN (15%), 4-NO2 (29%), 4-AcNH (38%), 3-AcNH (32%), 4-OMe (36%) and no significant difference (ns, p>0.05) was found statistically than PSMA-617 (25%). Still the derivatives show promising results and further research needs to be conducted to evaluate their uptake by the cells and their therapeutic image quality.

Subject Keywords

Antimicrobial peptides, Peptide hydrogels, Cannabidiol encapsulated peptide, PSMA peptides, Wound healing, Tumor-targeting peptides

URI

https://hdl.handle.net/11511/105297

Collections

Graduate School of Natural and Applied Sciences, Thesis