Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, anaerobic, ethanol-producing isolates

Date

2006-07-06

Author

Özkan, Melek
Özcengiz, Gülay

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In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, was found to differ for as much as 66 amino acid residues out of 100 (66%). The isolate 7-9-4 with a relatively low cellulose-degrading capacity also displayed amino acid variation for this protein, but only for 4 out of 118 residues. © TÜBİTAK.

Subject Keywords

Carbohydrate-binding modules, Cellulose degradation, Clostridium thermocellum, Thermophilic and cellulolytic bacteria

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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745645221&origin=inward
https://hdl.handle.net/11511/107064

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Department of Biology, Article