Lentil (Lens culinaris Medik)

Date

2015-01-01

Author

Akcay, Ufuk Celikkol
Yucel, Meral
Öktem, Hüseyin Avni

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This chapter describes an efficient Agrobacterium-mediated genetic transformation of lentil by use of cotyledonary node explants, an optimized wounding method, and vacuum infiltration. Transformation protocol was followed by direct regeneration of transgenic shoots and micrografting of the shoots on root stocks to obtain whole-plant regeneration. The most efficient transgene expression on the axil region was obtained when the Agrobacterium KYRT1 strain was used. Gradually increasing selection pressure and repeated removal of regenerated shoots between selection steps increased the number of transgene-expressing shoots greatly. This protocol allowed 2.3 % transformation efficiency and stable transgene expression and transmission which were tracked through three generations.

URI

https://hdl.handle.net/11511/107896

Journal

AGROBACTERIUM PROTOCOLS, VOL 1, 3RD EDITION

DOI

https://doi.org/10.1007/978-1-4939-1695-5_21

Collections

Department of Biology, Article

Citation Formats

U. C. Akcay, M. Yucel, and H. A. Öktem, “Lentil (Lens culinaris Medik),” AGROBACTERIUM PROTOCOLS, VOL 1, 3RD EDITION, vol. 1223, pp. 265–274, 2015, Accessed: 00, 2024. [Online]. Available: https://hdl.handle.net/11511/107896.