Opioid receptor labeling with the chloromethyl ketone derivative of [3H]Tyr-D-Ala-Gly-(Me)Phe-Gly-Ol (DAMGO) II: Covalent labeling of Mu opioid binding site by 3H-Tyr-D-Ala-Gly-(Me)Phe chloromethyl ketone

Date

1991-01-01

Author

Öktem, Hüseyin Avni
MOITRA, J
BENYHE, S
TOTH, G
LAJTHA, A
BORSODI, A

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Opioid receptors of rat brain membranes were prelabeled with H-3-Tyr-D-Ala2-(Phe4)-Gly-CH2Cl, a chloromethyl ketone derivative of enkephalin, and solubilized in 1% digitonin. Hydrodynamic parameters of the receptor detergent complex derived from gel filtration and sucrose density gradient ultra-centrifugation were found to be 51 A and 8.7 S, respectively, and the size was estimated to be about 200 kDa. Sodium dodecyl sulfate gel electrophoresis followed by fluorography revealed specific alkylation of a major protein at 58 kDa.

URI

https://hdl.handle.net/11511/107952

Journal

LIFE SCIENCES

DOI

https://doi.org/10.1016/0024-3205(91)90214-v

Collections

Department of Biology, Article

Citation Formats

H. A. Öktem, J. MOITRA, S. BENYHE, G. TOTH, A. LAJTHA, and A. BORSODI, “Opioid receptor labeling with the chloromethyl ketone derivative of [3H]Tyr-D-Ala-Gly-(Me)Phe-Gly-Ol (DAMGO) II: Covalent labeling of Mu opioid binding site by 3H-Tyr-D-Ala-Gly-(Me)Phe chloromethyl ketone,” LIFE SCIENCES, vol. 48, no. 18, pp. 1763–1768, 1991, Accessed: 00, 2024. [Online]. Available: https://hdl.handle.net/11511/107952.