Identification of SNP markers associated with resistance to Pyrenophora teres f. teres on barley

Date

2024-1-17

Author

Haydaroğlu, Ülkü Selcen

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Pyrenophora teres f. teres (Ptt) is the causal fungal agent of barley net-type net-blotch (NTNB) disease which can cause significant yield losses. In this study, phenotyping and genotyping data were obtained from a biparental barley doubled haploid (DH) mapping population of 277 DH lines inoculated with a virulent Ptt isolate (GPS18). The DH population was derived from the second-generation hybrids (F2) of a disease-resistant (Avcı 2002, “A”) and a susceptible (Bülbül 89, “B”) variety using anther culture technique. The pretreated anthers in 1.0 M mannitol showed statistically a better response than 0.7 M mannitol for A × B F2 hybrids. The callus induction ratio was significantly the highest at 37.6% for the “induction” medium, while the ratio of green plant formation was statistically the highest at 24.7% for the “R9” medium. Sequencing-based diversity array technology (DArT-seq) allowed the identification of 9,170 SNP markers, providing the construction of a linkage map of 1682.97 cM length, with an average density of the markers in 1.49 marker/cM. This led to the identification of three quantitative trait loci (QTLs) for Ptt on 3H, 4H, and 6H barley chromosomes. The QTL on the 6H was likely overlapping the previously reported SFNB-6H-33.74 locus, while the QTL on the 3H was potentially novel. On the other hand, the QTL on the 4H might be covering the Rpt7 locus. The percentage of phenotypic variation explained (PVE) by these QTLs was almost 26%, cumulatively. Disease-resistance-associated SNPs identified within these QTLs can be used for developing DNA tests.

Subject Keywords

Pyrenophora teres f. teres, Genetic mapping, DArT-seq, QTL, Barley

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https://hdl.handle.net/11511/108373

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Graduate School of Natural and Applied Sciences, Thesis