GOLD NANOROD-BASED SHAPE-SHIFTING PROBE FOR THE DETECTION OF HUMAN TELOMERIC G-QUADRUPLEX DNA STRUCTURE

Date

2024-1-17

Author

Özkan, Selin

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Colorimetric detection methods became one of the indispensable tools in biomedical research since they offer simple, rapid, visual, and often cost-effective means to identify specific biomolecular interactions. In this thesis, a novel colorimetric detection platform using gold nanorods (GNRs) for the detection of human telomeric G-quadruplex (Tel24 G4) DNA was introduced. The detection of Tel24 G4 is important due to the involvement of human telomeric DNA in vital cellular processes. The unique properties of longitudinal and transverse localized surface plasmon resonance (LSPR) exhibited by unmodified GNRs in cetyltrimethylammonium bromide (CTAB) solution serve as the foundation of the developed platform. Changes observed in the intensity and location of the longitudinal and transverse LSPR peaks of GNRs with increasing concentration of Tel24 G4 DNA suggested the aggregation and the etching as plausible mechanisms. Under the optimum conditions ([CTAB]=0.01 M, [HCl]=28 mM, 10 min incubation at 60℃), a linear dynamic range of 0.371 to 0.909 µM, with a detection limit of approximately 0.03 µM was determined. The platform was found to be highly specific to Tel24 G4 upon evaluation of its response to diverse oligonucleotide structures, encompassing other available G4 DNA configurations, single, double, and triple-stranded oligonucleotides. In brief, the developed platform has proven to be highly specific and selective to Tel24 G4 in addition to its simplicity, rapidity, and cost-effectiveness. We believe that the research conducted within this thesis not only advances the application potential of GNRs but also paves the way for their integration into the field of nucleic acid detection.

Subject Keywords

Altın nanoçubuklar= Gold nanorods, Prob= Probe, İnsan telomerik DNA'sı= Human telomeric DNA, G-dörtlü DNA= G-quadruplex DNA, Tespit= Detection

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https://hdl.handle.net/11511/108377

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Graduate School of Natural and Applied Sciences, Thesis