Melatonin loaded chitosan based hydrogels for cartilage regeneration

Date

2024-4-25

Author

Yeşilyurt, Büşra Nur

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

568
views

255
downloads

Cite This

Degeneration in the intervertebral discs (IVD) is a common worldwide health problem that is observed in most populations, especially in older age groups. It occurs as loss of disc height which causes severe pain and neurological disorders thus decreasing the quality of life. Tissue Engineering (TE) offers innovative and promising tools to restore, maintain, or improve the regeneration of intervertebral discs. In this thesis, an injectable chitosan (CS) /oxidized-maltodextrin (ox-MD) hydrogel reinforced with oxidized microcrystalline cellulose (ox-MCCs) was developed with the ultimate aim of treatment of degenerated intervertebral discs. Melatonin (MEL) which helps to protect the structural integrity of IVD structure was also incorporated into the hydrogels. Different final concentrations of CS (1.5%, 2%, 2.5%), and ox. MD (0.5%, 0.25%, 0.125%) in the hydrogel compositions were investigated in terms of gelation time, degradation, injectability, and water uptake. Considering these properties 2%CS-0.5%ox.MD hydrogel was chosen for MCC incorporation and melatonin loading experiments. Different amounts of ox-MCC (0.5%, 0.25%, 0.125%) were incorporated into these hydrogels. MCC addition significantly decreased the gelling time (33 to 12 seconds) in proportion to the increase in MCC. v Besides that at the highest MCC-containing composition hydrogels could retain their weight up to 70-75% compared to 40% without MCC. Additionally, increasing ox MCC content increased the Young Moduli of the hydrogels (the highest MCC content improved the Young Modulus by approximately 6 times). Melatonin release from the hydrogels with and without ox-MCC reached 35.29 % ± 2.3 and 26.65 % ±3.7, respectively at the end of 3 days. Cell culture studies conducted with nucleus pulpous (NP) cells isolated from bovine vertebral tissue showed that hydrogel groups had no toxic effects on cells and they supported proliferation at days 3 and 7 in melatonin-loaded groups. Therefore, melatonin-loaded (CS%2-ox.MD%0.5 ox.MCC%0.5) hydrogels may hold promise for use in the regeneration of IVD.

Subject Keywords

Injectable hydrogels, Cartilage tissue engineering, Intervertebral disc (IVD) degeneration, Melatonin, Chitosan

URI

https://hdl.handle.net/11511/109830

Collections

Graduate School of Natural and Applied Sciences, Thesis