Application of CRISPR/Cas9 Pooled Screening for a Non-immediate Readout Model

Date

2024-01-01

Author

Terzi Çizmecioğlu, Nihal

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Linking phenotypes to genetic components has been an essential part of novel drug discovery, and screening methods have been widely employed to achieve such a goal. Screens can be conducted in either pooled or arrayed formats. Although arrayed screenings provide a better and cheaper alternative in small scale, the larger-scale screenings are conducted in pooled manner. With its adaptability to various models and conditions, CRISPR/Cas9 technology provides an invaluable alternative to classical and RNAi-based screening methods. Combined with high-throughput sequencing and bioinformatics, CRISPR-/Cas9-based pooled screening methods provide unbiased and robust data. In this protocol, we employed CRISPR-/Cas9-based pooled screening for a non-binary and non-immediate readout.

Subject Keywords

Cas9, CRISPR, Differentiation, Neuroectoderm, Pooled screening, Sox1GFP

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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85199224188&origin=inward
https://hdl.handle.net/11511/110736

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Department of Biology, Article