Studies towards zinc(II) bisdipicolylamine-bearing particles for the sequestration, agglutination, and inhibition of the bacteria

Date

2024-7

Author

Yılmaz, Ozan

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Thousands of patients die every year all around the world due to the emergence of antibiotic resistance, and almost half of these deaths are caused by bacteremia. Antibiotic resistance is the ability of bacteria to survive in the presence of cytotoxic (bactericidal) or growth-inhibitory (bacteriostatic) antibiotics. To overcome antibiotic resistance, it is necessary to improve currently available therapeutic strategies or develop new ones. Finding a general solution for antibiotic resistance requires universal bacterial targeting. In this context, Zn(II) bisdipicolylamine (Zn2BDPA) complexes are very important, as they are capable of selectively targeting negatively charged phosphate amphiphiles in biomembranes, including those of bacterial cells. This study explored the improvement of currently available strategies and the development of new ones for antibacterial diagnosis/therapy. In this context, the first approach was to synthesize bacterial targeting elements and conjugate them with oleic acid to be part of bacterial targeting liposomes. The second approach involved preparing bacterial targeting liposomes (Lipo1 and Lipo4) and investigating their bacterial agglutination potential. The third approach involved the preparation of bacterial-targeting cellulose microspheres (BDPA-CMs) for the sequestration of bacteria from aqueous media and exploring their bacterial binding capacities in buffer and blood media. The results showed that Lipo1/4 have selective agglutination properties towards negatively charged liposomes and bacterial cells. Agglutination was investigated both quantitatively and qualitatively. BDPA-CMs exhibited robust affinity towards bacterial cells in buffer and blood media and were not hemolytic. These findings may lead to novel strategies for the diagnosis and treatment of bacterial infections.

Subject Keywords

Antibiotic resistance, Zinc(II) BDPA complexes, Agglutination, Bacterial sequestration

URI

https://hdl.handle.net/11511/110869

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Graduate School of Natural and Applied Sciences, Thesis