Boron reinforced fibroin membrane for periosteal tissue engineering

Date

2024-8-14

Author

AKBABA FATHI, SEMA

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

334
views

755
downloads

Cite This

In this thesis, novel fibroin/sulfated alginate (F/sA) membranes loaded with exosomes of human adipose derived stem cell (hADSC) treated with pure and doped hydroxyapatite (HA) were developed for periosteal tissue engineering. It was found that, co-doping of B (8 mol%) and Zn (4 mol%) to HA provided both proliferative and osteogenic effects on hADSCs and angiogenic effect on human umbilical vein endothelial cells. Both F and sA led to immunomodulation by significantly decreasing IL1B and CASP1 levels of THP-1 macrophages. However, presence of sA more than 5% did not cause significant change. Considering that cell viability and proliferation were the highest for 95:5 group, 95% F to 5% sA was chosen as the optimum ratio. Finally, effects of untreated and HA, 8B HA and 8B 4Zn HA treated exosomes were investigated. 8B HA group significantly increased exosome yield whereas treatment with all HA groups significantly decreased protein and DNA levels. Moreover, treatment with doped HA led to significant increase of RNA levels. F/sA membranes loaded with exosome groups increased early attachment and proliferation of hADSCs. Treated exosome groups were found to increase osteogenic differentiation and decreased expression of inflammatory markers except for 8B 4Zn HA group, which increased CASP1. Overall, F/sA membrane loaded with exosomes isolated from cells treated with pure and doped HA groups were able to increase proliferation and osteogenic differentiation of hADSCs as well as immunomodulation. Further identification of exosomes isolated from differently treated cells would bring a better understanding for their effects and potential uses.

Subject Keywords

Tissue Engineering, Hydroxyapatite, Exosome, Periosteum, Boron

URI

https://hdl.handle.net/11511/111044

Collections

Graduate School of Natural and Applied Sciences, Thesis