Synthesis and design of functional fullerenol-based electrochemical nanobiosensor to examine the inhibition effects of anti-Alzheimer drug active pyridostigmine on acetylcholinesterase

Date

2025-09-01

Author

Soyler, Dilek
Dolgun, Volkan
KURBANOĞLU, SEVİNÇ
Özçubukçu, Salih
Soylemez, Saniye

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Glutamic acid functionalized fullerenol (F-Glu) based electrochemical AChE enzyme biosensor (GE/F-Glu/AChE) was utilized for the detection of acetylthiocholine chloride and pyridostigmine for the first time. The performance of the biosensor was validated, including recovery studies on human serum and urine, to assess method accuracy. The GE/F-Glu/AChE biosensor exhibited a Limit Of Detection (LOD) of 0.0004 mM and a Limit Of Quantification (LOQ) of 0.001 mM for acetylthiocholine chloride detection. Morphological and electrochemical characterizations of the biosensors were conducted using FT-IR, FE-SEM, AFM, CV, and EIS techniques. Furthermore, the biosensor was employed to investigate the inhibition of AChE activity by Pyridostigmine (PDT), with an IC50 value of 1.01 mM PDT. The inhibitory effect of PDT on the biosensor was explored in synthetic human serum, synthetic human urine, and pharmaceutical formulations containing PDT's active ingredient under optimal biosensor conditions.

Subject Keywords

Acetylcholinesterase, Electrochemical biosensor, Fullerenol, Inhibition, Pyridostigmine

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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105002326531&origin=inward
https://hdl.handle.net/11511/114319

Collections

Department of Chemistry, Article