CHARACTERIZING NOVEL RNA EDITING SITES IN THE 3'UTR OF F11R IN BREAST CANCER

Date

2025-4-7

Author

Karagözoğlu, Deniz

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ADAR enzymes catalyze adenosine-to-inosine (A-to-I) RNA editing, a crucial post-transcriptional modification that shapes the epitranscriptome. By changing RNA stability, splicing, and translation, this alteration substantially affects gene expression, which in turn affects protein levels and functions. Although dysregulated RNA editing has been connected to several conditions, including cancer, deciphering individual RNA editing cases is still needed. This study focuses on RNA editing events in the 3′ untranslated region (UTR) of F11R (Junctional Adhesion Molecule A (JAM-A)), a gene implicated in breast cancer. Leveraging in-silico predictions from TCGA breast cancer datasets, candidate editing sites were identified for F11R. Given the differential mRNA and protein expression of F11R across breast cancers, we examined RNA editing of F11R in breast cancer cell models. Our experimental results confirmed multiple RNA editing points on F11R 3’UTR and the involvement of ADAR1 in these editing events. We further provide initial insight into the functionality of these editing sites for F11R protein levels. Revealing RNA editing of 3’UTRs may help us better understand the post-transcriptional regulation of gene expression.

Subject Keywords

RNA Editing, Breast Cancer, ADAR1, F11R

URI

https://hdl.handle.net/11511/114492

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Graduate School of Natural and Applied Sciences, Thesis