Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001

Date

2020-7-15

Author

KARS, Gökhan
DEMİREL KARS, Meltem
OBALI, İhsan
EMSEN, Ayça
Gündüz, Ufuk

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Carotenoids take special attention due to their strong antioxidant properties. In the present study, the antioxidant and antiproliferative effects of carotenoids extracted from Rhodobacter sphaeroides O.U. 001 were investigated. For this, R. sphaeroides O.U. 001 was first cultivated in a 5 L bioreactor. After that, the carotenoids were extracted from R. sphaeroides O.U. 001 using acetone as solvent. The effects of carotenoid extract on proliferation of vincristine and paclitaxel resistant cells (MCF-7/Vinc, MCF-7/Pac) were investigated by MTT cytotoxicity assay. The checkerboard plate technique was performed to evaluate the interactions between carotenoid extract and cancer drugs on the cells. The antioxidant activity of the carotenoid extract was assessed by 2, 2 - diphenyl, 1- picryl hydrazyl (DPPH) radical scavenging assay. As a result of extraction, considerable amount of carotenoid yield was obtained (80.69 mg carotenoid/g dry cell weight). It was found that carotenoid extract had no inhibitory effects on cell proliferation and interaction of carotenoids with anticancer drugs exhibited antagonistic effects. In addition to these, the DPPH antioxidant activity (IC50 value) of the carotenoid extract was calculated as 25 µg/mL. In conclusion, biotechnologically produced bacterial carotenoids were shown to have considerable antioxidant activity without any cytotoxicity on cancer cells.

Subject Keywords

Antioksidan, Biyoreaktör, Karotenoid, MCF-7, Rhodobacter sphaeroides, Antioxidant, Bioreactor, Carotenoid, MCF-7, Rhodobacter sphaeroides

URI

https://dergipark.org.tr/tr/pub/gumusfenbil/issue/55135/597048
https://hdl.handle.net/11511/115033

Collections

Department of Biology, Article