The synthesis of Polylysine-Fullerenol conjugates and their applications as biosensors

Date

2025-8-29

Author

Sözer, Yasin

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In recent years, nanoparticle fullerenol has emerged as a prime candidate for biosensor applications when functionalized with biomolecules. While retaining many of its precursor fullerene’s desirable traits such as physical prowess, electrical sensitivity and conductivity, fullerenols are better suited for many biomedical applications due to their increased solubility in aqueous media. An established biomolecule in biomedical applications, α-poly-L-lysine, known for its antimicrobial, coating properties, was chosen to functionalize fullerenol. Polylysine having a positive charge and a free primary amine on its side chain promotes many molecular interactions. This bionanoconjugate is investigated as a biosensor. To this end, Fluorescein isothiocyanate is used to fluorescently label the conjugate, the end product becoming a basis for further modifications. While this is not the primary focus of the thesis, it is presented more as a proof of concept that shows the nanoconjugate system’s versatility. The main goal is to showcase the sensitivity this system has as a biosensor by linking the conjugate with GOx using glutaraldehyde. GOx is an oxidoreductase enzyme that has become the gold standard for the accurate biosensing of glucose concentrations. After looking at the literature it was seen that there is yet to be a biomolecule of big scale and versatility in terms of bridging others, conjugated to fullerenol as PLL. While setting the conjugate to be a biosensing system, a novelty unique to this design, the in-solution linking of the enzyme, is compared with the conventional method and the overall results analyzed.

Subject Keywords

Fullerenol, FITC, Glucose oxidase, α-Poly-L-lysine, Biosensor

URI

https://hdl.handle.net/11511/116107

Collections

Graduate School of Natural and Applied Sciences, Thesis