Forging the Sila-Resorufin Probe: A New Light in Quest for Cancer Treatment

Date

2025-8-28

Author

Çakmak, Ezgi

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Fluorescence imaging is a sensitive method widely used in the study of biological systems at the molecular level. Photodynamic therapy (PDT) is considered a minimally invasive cancer therapy that provides treatment by causing oxidative damage to target cells through light-sensitive agents. The effectiveness of both methods depends on the development of small molecular photosensors with suitable optical and chemical properties. Xanthene-based dyes are among the important candidates in this field due to their high molar absorption coefficient, photostability and tunable structural properties. In this study, we focused on silicon-containing derivatives of Xanthene and developed new designs based on the precursor molecule called SiNO, which was obtained by sp² carbon with nitrogen and oxygen modification with dimethyl silane of 2-methyl Tokyo Magenta. The low fluorescence quantum yield of SiNO limits its use for both imaging and therapeutic purposes. Therefore, structural modifications aimed at improving optical efficiency were carried out by following the strategies reported by Choi et al. The SiNO-Me designed for fluorescence imaging applications by adding methyl groups to the ortho position of nitrogen; SiNO-X, which has integrated halogen groups, is designed for photodynamic therapy applications. Studies on the synthesis of both molecules are ongoing, and the structure of SiNO-Me has been confirmed by high-resolution mass spectrometry (HRMS); however, it has not yet been isolated in pure form. These two structures are evaluated as holistic treatment and diagnostic methods in different biomedical applications. Synthesis optimizations and photophysical characterization studies are ongoing.

Subject Keywords

Fluorescence Imaging, Photodynamic Therapy,, Photosensitizer

URI

https://hdl.handle.net/11511/116147

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Graduate School of Natural and Applied Sciences, Thesis