Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source "Turkish DeLight"

Date

2023-01-01

Author

Atalay, Necati
Akcan, Enver Kamil
Gul, Mehmet
Ayan, Esra
Destan, Ebru
Ertem, Fatma Betuel
Tokay, Nurettin
Cakilkaya, Baris
Nergiz, Zelis
Karakadioglu, Gozde
Kepceoglu, Abdullah
Yapici, Ilkin
Tosun, Bilge
Baldir, Nilufer
Yildirim, Gunseli
Johnson, J. Austin
Guven, Omur
Shafiei, Alaleh
Arslan, Nazli Eylul
Yilmaz, Merve
Kulakman, Cahine
Paydos, Seyide Seda
Cinal, Zeynep Sena
Sabanoglu, Kardelen
Pazarceviren, Aysegul
Yilmaz, Aysenur
Canbay, Basak
Asci, Bengisu
Kartal, Esra
Tavli, Serra
Caliseki, Mehmet
Goc, Gunce
Mermer, Arif
Yesilay, Gamze
Altuntas, Sevde
Tateishi, Hiroshi
Otsuka, Masami
Fujita, Mikako
Tekin, Saban
Ciftci, Halilibrahim
Durdagi, Serdar
Doganay, Gizem Dinler
Karaca, Ezgi
Turkoz, Burcu Kaplan
Kabasakal, Burak Veli
Kati, Ahmet
Demirci, Hasan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

7
views

0
downloads

Cite This

X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Turkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, Istanbul, Turkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.

URI

https://hdl.handle.net/11511/117127

Collections

Department of Biology, Article