Selection of DNA-Encoded Libraries to Protein Targets within and on Living Cells

Date

2019-10-01

Author

Cai, Bo
Kim, Dongwook
Akhand, Saeed
Sun, Yixing
Cassell, Robert J.
Alpsoy, Aktan
Dykhuizen, Emily C.
Van Rijn, Richard M.
Wendt, Michael K.
Krusemark, Casey J.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

26
views

9
downloads

Cite This

We report the selection of DNA-encoded small molecule libraries against protein targets within the cytosol and on the surface of live cells. The approach relies on generation of a covalent linkage of the DNA to protein targets by affinity labeling. This cross-linking event enables subsequent copurification by a tag on the recombinant protein. To access targets within cells, a cyclic cell-penetrating peptide is appended to DNA-encoded libraries for delivery across the cell membrane. As this approach assesses binding of DELs to targets in live cells, it provides a strategy for selection of DELs against challenging targets that cannot be expressed and purified as active.

URI

https://hdl.handle.net/11511/117326

Collections

Department of Biology, Article