Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Isolation, characterization and immobilization of polyphenol oxidases from mulberry(morus alba) leaf tissues
Download
index.pdf
Date
2003
Author
Sutay, Didem
Metadata
Show full item record
Item Usage Stats
245
views
97
downloads
Cite This
In this study, the aim was to find an economical plant source for polyphenol oxidase (PPO) production as an alternative to mushroom and possible application areas by characterization and immobilization of the PPOs. For this purpose, tissues of various plants of no commercial value were screened for their PPO activities. Mulberry leaf tissues showed the highest PPO activity against 4-methyl catechol which was comparable to that of mushroom. Average Km and Vmax values of free mulberry leaf PPOs were found as 7 mM and 218 U/ml, respectively. Mulberry leaf PPOs were immobilized in a polypyrole matrix and the Km and Vmax values of immobilized PPOs were calculated as 35 mM and 3 U/ml, respectively. Mulberry leaf PPO was the most active at 45°C and pH 7. By using electrophoretic analysis, laccase and catechol oxidase type activities of PPOs and in addition, peroxidase activity were detected. Molecular weights of laccase, peroxidase and catechol oxidase were found to be about 62, 64 and 62-64 kDa, with pI values of 8.0-8.5, 4.5 and 10, sequentially.
URI
http://etd.lib.metu.edu.tr/upload/1262955/index.pdf
https://hdl.handle.net/11511/13627
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Identification and characterization of hydrolytic enzymes from the midgut of Sunn Pest of wheat (Eurygaster integriceps)
Ogur, E.; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Informa UK Limited, 2009-01-01)
To help in the development of Sunn Pest-resistant transgenic plants employing protease or alpha-amylase inhibitors, midgut hydrolytic enzymes of Sunn Pest (Eurygaster integriceps, Put.) (Heteroptera: Scutelleridae) were identified and characterized biochemically. We observed levels of very low proteolytic activity of trypsin (3 nmoles/min/mg), elastase (0.66 nmoles/min/mg) and leucine aminopeptidase-like (14.4 nmoles/min/mg) proteases, but no chymotrypsin and papain-like activity. Proteolytic activities wer...
Removal of trihalomethane precursors by granular activated carbon treatment
CAPAR, Goksen; Demirer, Göksel Niyazi; Dilek, Filiz Bengü; Yetiş, Ülkü (2001-02-01)
This research covers the determination of the existing situation of trihalomethane (THM) levels in the distribution system of Ankara City and also focuses on the investigation of effectiveness of granular activated carbon (GAC) adsorption for the removal of natural organic matter (NOM) that are THM precursors and also THMs. For this purpose, a survey was carried out at 14 selected points in the city and the THMs were observed to be present in the Ivedik Water Treatment Plant (IWTP) filter effluent and in th...
Removal of Pesticides from Wastewater by Membrane Proces
Ateş, Nuray; Uzal, Niğmet; Yetiş, Ülkü; Dilek, Filiz Bengü (2021-06-23)
Purpose: The aim of this study was to investigate the removal performances of four pesticides (tributyl phosphate, flutriafol, dicofol and irgarol) by RO membranes. Methods: Three different RO membranes (BW30-LE, SW30-XLE and GE-AD) were used to reject pesticides in two transmembrane pressures of 10 and 20 bar in bench-scale membrane filtration cell. Tributyl phosphate and flutriafol were detected by GC/MS and dicofol and irgarol were monitored by HLPC instruments. Results: While the highest removal of trib...
TREATMENT OF EFFLUENTS FROM HEMP-BASED PULP AND PAPER-INDUSTRY .1. WASTE CHARACTERIZATION AND PHYSICOCHEMICAL TREATABILITY
Dilek, Filiz Bengü (1994-01-01)
This study was conducted to determine the wastewater characteristics as well as the treatment alternatives for the Tafkopru Pulp and Paper Mill in Turkey. The physico-chemical treatability study was conducted by standard Jar-test equipment. The highest COD removal efficiency obtained was 96% for the paper machine, 50% for the alkali extraction and 20% for the bleaching effluents by using alum as coagulant. The colour removal efficiency was around 80% for the alkali extraction effluents.
Treatment of Effluents from Hemp Based Pulp and Paper Industry I Waste Characterization and Physico chemical Treatability
Dilek, Filiz Bengü (1993-10-15)
This study was conducted to determine the wastewater characteristics as well as the treatment alternatives for the Tafkopru Pulp and Paper Mill in Turkey. The physico-chemical treatability study was conducted by standard Jar-test equipment. The highest COD removal efficiency obtained was 96% for the paper machine, 50% for the alkali extraction and 20% for the bleaching effluents by using alum as coagulant. The colour removal efficiency was around 80% for the alkali extraction effluents. This study was cond...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
D. Sutay, “Isolation, characterization and immobilization of polyphenol oxidases from mulberry(morus alba) leaf tissues,” M.S. - Master of Science, Middle East Technical University, 2003.
