Production of tannese by Aspergillus niger

Download
index.pdf
2006
Öztürk, Alev Deniz
Show full item record
150
views
43
downloads
In this study, a filamentous fungus, Aspergillus niger was evaluated in terms of extracellular tannase production. The effect of tannic acid, glucose and nitrogen sources on tannase and biomass productions was investigated and their concentrations were optimized. The highest enzyme activity was recorded as 316 U/ml in the optimized medium containing 8% Tannic acid, 1% Glucose, 0.4% (NH4)2HPO4, 0.1% K2HPO4, 0.1% MgSO4.7H2O, 0.01% ZnSO4.7H2O, 0.0005% NaCl in a shake-flask bioreactor at 35oC and 175 rpm. The bioreaction profile including tannic acid, gallic acid, pyrogallol, glucose concentrations, pH, biomass and extracellular tannase production were determined under the optimized conditions. The maximum extracellular tannase activity (316 U/ml) was observed on the 4th day of cultivation. However, biomass continued to increase up to the 9th day of fermentation. Increase in biomass concentration during the first two days and after the 7th day was high. The microorganism used tannic acid and glucose during the first two days by considering the sharp decrease in tannic acid and glucose concentrations. The increase in biomass concentration after the 7th day was directly proportional to the decrease in pyrogallol concentration in this period of time. The pH of the cultivation medium decreased from 5.5 to 2.3 owing to the assimilation of glucose and the production of gallic acid. Keywords: Tannase, Aspergillus niger, Enzyme production, Cultivation profile, Tannic acid.
Chemical Engineering.
http://etd.lib.metu.edu.tr/upload/3/12607444/index.pdf
https://hdl.handle.net/11511/16394
Graduate School of Natural and Applied Sciences, Thesis

Suggestions

Effect of pH on erythropoietin production by recombinant Pichia pastoris in fed-batch operation
Soyaslan, Elif Şükran; Çalık, Pınar; Department of Chemical Engineering (2010)
In this study, the effects of pH on therapeutically important protein, recombinant human erythropoietin (rhuEPO), production by Pichia pastoris was investigated at pH=4.0, 4.5, 5.0, 5.5 and 6.0. rHuEPO production was started by methanol induction in fed-batch mode. The highest cell concentration was obtained at pH=4.5 as 81.4 g L-1. The co-substrate substrate sorbitol, which was added batch-wise, was consumed at t=15 h of the operations at pH=4.0, 4.5 and 5.0. However as the pH increases above pH=5.0 the so...
Dynamic and steady-state analysis of oxidative dehydrogenation of ethane
Karamullaoğlu, Gülsün; Doğu, Timur; Department of Chemical Engineering (2005)
In this research, oxidative dehydrogenation of ethane to ethylene was studied over Cr-O and Cr-V-O mixed oxide catalysts through steady-state and dynamic experiments. The catalysts were prepared by the complexation method. By XRD, presence of Cr2O3 phase in Cr-O; and the small Cr2O3 and V2O4 phases of Cr-V-O were revealed. In H2-TPR, both catalysts showed reduction behaviour. From XPS the likely presence of Cr+6 on fresh Cr-O was found. On Cr-V-O, the possible reduction of V+5 and Cr+6 forms of the fresh sa...
Flow sheet optimization by the concept of sustainable development: alumina industry
Kurucak, Abdurrahman; Gürüz, Güniz; Department of Chemical Engineering (2010)
In this study, effects of changes in various parameters of the Bayer process on the amount of “red mud”, which has many environmental drawbacks, were examined in accordance with the principles of “sustainable development”. The production process of Seydişehir Aluminum Plant is modeled as a case study. First a steady-state mass balance calculation is carried out by incorporating sequential modular approach. Then a model of the Bayer process digester is programmed and several simulations are carried out using...
Chiral seperations by enzyme enhanced ultrafiltration : fractionation ofracemic benzoin
Ölçeroğlu, Ayşe Hande; Yılmaz, Levent; Department of Chemical Engineering (2006)
In this study, a methodology for separation of chiral molecules, by using enhanced ultrafiltration system was developed. Benzoin was the model chiral molecule studied. In the scope of developing this methodology, some parameters were investigated in the preliminary ultrafiltration experiments in order to set the operation conditions for enhanced ultrafiltration experiments. Due to the slight solubility of benzoin in pure water, 15% (v/v) Polyethylene glycol (PEG 400) and 30 % (v/v) Dimethyl sulfoxide (DMSO)...
Bioprocess development for therapeutical protein production
Çelik Akdur, Eda; Çalık, Pınar; Department of Chemical Engineering (2008)
In this study, it was aimed to develop a bioprocess using the Pichia pastoris expression system as an alternative to the mammalian system used in industry, for production of the therapeutically important glycoprotein, erythropoietin, and to form stoichiometric and kinetic models. Firstly, the human EPO gene, fused with a polyhistidine-tag and factor-Xa protease target site, in which cleavage produces the native termini of EPO, was integrated to AOX1 locus of P. pastoris. The Mut+ strain having the highest r...
Citation Formats
A. D. Öztürk, “Production of tannese by Aspergillus niger,” M.S. - Master of Science, Middle East Technical University, 2006.
METU IIS - Integrated Information Service open@metu.edu.tr