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Screening of tomato seeds for genetic modification and identification of genetically modified ripening delayed tomato seeds

Türkoğlu, Selda
Tomato has been genetically modified for providing properties such as insect-resistance or delayed-ripening. Tomato seeds purchased from several bazaars and markets were screened for the presence of genetic modification by targeting NptII kanamycin resistance, Nos terminator, and 35S promoter gene regions which are the most commonly transformed gene regions in transgenic plants, and then ripening-delayed tomato seeds were tried to be identified in this study. F type truncated-PG gene and Sam-k gene were selected as the indicator of genetically modified ripening delayed tomatoes. DNAs of 25 seed samples were isolated by CTAB method and examined with several primer pairs, and the primer sets that provided consistent results were selected to conduct routine testing by PCR analysis of the samples. In screening analysis via conventional PCR, 4 samples were amplified with 35S, Nos and NptII primer sets. Among other samples, 3 of them were amplified with 35S and Nos primer sets and 2 of them were amplified only with 35S primer set. The amplification was observed with Nos, NptII and Sam-k primers in one sample and this sample was identified as 35 1 N, since the sequence result of the PCR product amplified with Sam-k primers showed high homology with the Samase gene of T3 Coliphage. F type truncated PG gene was not observed in any of the samples. Although this study demonstrates the presence of commonly used gene regions in genetically modified tomatoes, further analysis of the genetically modified ripening delayed tomato seeds via construct specificor event specific PCR techniques is needed for confirmation.