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Production, Purification and characterization of chitosanase from Penicillium spinulosum
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068782.pdf
Date
1997
Author
Ak, Özlem
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https://hdl.handle.net/11511/1957
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Graduate School of Natural and Applied Sciences, Thesis
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Production, purification and characterization of chitosanase from Penicillium spinulosum
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A chitosan degrading fungus which had been isolated in our laboratory and identified as Penicillium spinulosum by the International Mycological Institute (England), was used for the production of chitosanase in a salt medium containing Rhizopus cell walls as the sole carbon source. Although chitosanase was produced under all the conditions tested about 1 % cell wall concentration maximized enzyme production. The enzyme was purified 50 fold by using ammonium sulfate precipitation and ion-exchange chromatogra...
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Ö. Ak, “Production, Purification and characterization of chitosanase from Penicillium spinulosum,” Middle East Technical University, 1997.