Role of tnf-alpha in skeletal muscle atrophy in ovariectomized rats : an experimental functional, histological and molecular biology study

Download

index.pdf

Date

2010

Author

Dağdeviren, Sezin

Metadata

Show full item record

Item Usage Stats

178
views

354
downloads

Skeletal muscle is defined to be atrophic in osteoporosis models and therefore is a potential target tissue for osteoporosis research. The aim of this longitudinal randomized controlled interdisciplinary study was to analyze the functional, histological, ultra-structral and molecular changes and the role of cachectic muscle atrophy inducer TNF-alpha in the skeletal muscles of the ovariectomized (OVX) rat model which mimics postmenopausal osteoporosis. Female Sprague-Dawley rats were randomly assigned to the control, the OVX and the OVX+10μg/g/week TNF-alpha antagonist (Remicade) treated OVX-TNF groups. Maximum isometric and tetanic-twitch amplitudes were lower than the control group in the OVX group. Maximum isometric twitch amplitudes recovered in the fast-twitch extensor digitorum longus (EDL) muscles but not in the slow-twitch soleus muscles in the OVX-TNF group. The decrease in tetanic-twitch amplitudes recovered in the OVX-TNF group in both muscle types. Splitting and size variations of fibers, central nuclei and well-preserved overall ultrastructure were noted in the OVX and the OVX-TNF groups. Slow-twitch Type I fiber percentage, areas and diameters increased in EDL muscles of the OVX and the OVX-TNF group comparing to the control group. p65 and MyoD immune-labeling increased in OVX group whereas MyoD and C-Rel increased and p50 decreased in OVX-TNF group. Expressions of 61 genes and 42 unidentified transcripts were significantly different between the control, the OVX and the OVX-TNF groups. To sum up TNF-alpha has a role in skeletal muscle dysfunction in OVX rats and TNF-alpha antagonist administration recovered it. But this modulation was not sufficient for total structural recovery.

Subject Keywords

Biotechnology., Osteoporosis., Skeletal muscle.

URI

http://etd.lib.metu.edu.tr/upload/3/12612112/index.pdf
https://hdl.handle.net/11511/19702

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Effect of curvature on lower extremity residual limb models Tönük, Ergin (null; 1999-12-01) Indentor tests are one of the most common ways for in vivo estimation of human residual limb soft tissue bulk elastic and viscoelastic material properties. Due to large strains and displacements encountered during indentation, elementary strength of materials approaches yield crude approximations of the material properties. A common procedure, therefore, is to construct a finite element model of the tissue in the vicinity of the test site. The material properties of soft tissue are identified by matching th...
The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study Bozkurt, Ozlem; Bilgin, Mehmet Dincer; Severcan, Feride (Hindawi Limited, 2007) Diabetes mellitus (DM) is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by a defective insulin secretory response. Skeletal muscle takes role in determination of carbohydrate and lipid metabolism, therefore; it is one of the target tissues of diabetes. Herein this study, application of Fourier Transform Infrared (FTIR) spectroscopy in diabetic skeletal Extensor Digitorum Longus (EDL) muscle tissues will be presented which highlight the promise of this technique in me...
Influence of co-culture on osteogenesis and angiogenesis of bone marrow mesenchymal stem cells and aortic endothelial cells Pekozer, Gorke Gurel; KÖSE, GAMZE; Hasırcı, Vasıf Nejat (2016-11-01) Co-culture of bone forming cells and endothelial cells to induce pre-vascularization is one of the strategies used to solve the insufficient vascularization problem in bone tissue engineering attempts. In the study, primary cells isolated from 2 different tissues of the same animal, rat bone marrow stem cells (RBMSCs) and rat aortic endothelial cells (RAECs) were co-cultured to study the effects of co-culturing on both osteogenesis and angiogenesis. The formation of tube like structure in 2D culture was obs...
Relationship of Epicardial Adipose Tissue Thickness with Early Indicators of Atherosclerosis and Cardiac Functional Changes in Obese Adolescents with Metabolic Syndrome Bedir, Akyol; Mehmet, Boyraz; Cevriye, Aysoy (Galenos Yayinevi, 2013-9-5) Objective: Epicardial adipose tissue thickness (EATT) is suggested as a new cardiometabolic risk factor. Carotid intima-media thickness (IMT) is a potential indicator of subclinical atherosclerosis in patients with metabolic syndrome (MS). We investigated the association of EATT with carotid IMT and cardiac functional changes in obese adolescents with MS. Methods: One hundred thirty-eight obese adolescents and 63 lean subjects were enrolled in the study. The obese subjects were divided into two subgroups...
Structural alterations in rat liver proteins due to streptozotocin-induced diabetes and the recovery effect of selenium: Fourier transform infrared microspectroscopy and neural network study Bozkurt, Ozlem; Bayari, Sevgi Haman; Severcan, Mete; Krafft, Christoph; Popp, Juergen; Severcan, Feride (2012-07-01) The relation between protein structural alterations and tissue dysfunction is a major concern as protein fibrillation and/or aggregation due to structural alterations has been reported in many disease states. In the current study, Fourier transform infrared microspectroscopic imaging has been used to investigate diabetes-induced changes on protein secondary structure and macromolecular content in streptozotocin-induced diabetic rat liver. Protein secondary structural alterations were predicted using neural ...

Citation Formats

S. Dağdeviren, “Role of tnf-alpha in skeletal muscle atrophy in ovariectomized rats : an experimental functional, histological and molecular biology study,” M.S. - Master of Science, Middle East Technical University, 2010.