Flow injection fluorescence determination of dopamine using a photo induced electron transfer (PET) boronic acid derivative

Date

2005-8

Author

Ebru Seçkin, Z.
Volkan, Mürvet

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An automated flow injection analysis system was developed for the fluorometric determination of dopamine in pharmaceutical injections. The method is based on the quenching effect of dopamine on m-dansylaminophenyl boronic acid (DAPB) fluorescence due to the reverse photo induced electron transfer (PET) mechanism. Effects of pH and interfering species on the determination of dopamine were examined. Calibration for dopamine, based on quenching data, was linear in the concentration range of 1.0 x 10(-5) to 1.0 x 10(-4) M. Detection limit (3s) of the method was found to be 3.7 x 10(-6) M. Relative standard deviation of 1.2% (n = 10) was obtained with 1.0 x 10(-5) M dopamine standard solution. The proposed method was applied successfully for the determination of dopamine in pharmaceutical injection sample. The sampling rate was determined as 24 samples per hour.

Subject Keywords

Fow injection analysis (FIA), Photo induced electron transfer (PET), Spectrofluorometry, Boronic acid, Dopamine, Injection

URI

https://hdl.handle.net/11511/28545

Journal

Analytica Chimica Acta

DOI

https://doi.org/10.1016/j.aca.2005.02.037

Collections

Department of Chemistry, Article

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Citation Formats

Z. Ebru Seçkin and M. Volkan, “Flow injection fluorescence determination of dopamine using a photo induced electron transfer (PET) boronic acid derivative,” Analytica Chimica Acta, pp. 104–108, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/28545.