Conductometric enzyme biosensors based on natural zeolite clinoptilolite for urea determination

Saiapina, O. Y.
Pyeshkova, V. M.
Soldatkin, O. O.
Melnik, V. G.
Akata Kurç, Burcu
Walcarius, A.
Dzyadevych, S. V.
Jaffrezic-Renault, N.
Highly sensitive conductometric urea biosensors were developed by exploiting the successful combination of ammonium-sieving and ion exchange properties of clinoptilolite, with a unique biorecognition capacity of urease. To optimize the performance of urea biosensors based on clinoptilolite, the dependences of their analytical signals on pH, buffer capacity and ionic strength of phosphate buffer solution (PBS) were studied. Optimum pH for urea biosensors was found within the range of pH 6.0-7.0. The dependences of biosensors responses on buffer capacity and ionic strength of PBS were of the same profile as those obtained for the urea biosensor which was not modified with clinoptilolite.


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Kirdeciler, Salih Kaan; Soy, Esin; Öztürk, Seçkin; Kucherenko, Ivan; Soldatkin, Oleksandr; Dzyadevych, Sergei; Akata Kurç, Burcu (2011-09-15)
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Polylactic acid (PLA) is a widely used bioresorbable polymer in medical devices owing to its biocompatibility, bioresorbability, and biodegradability. It is also considered a sustainable solution for a wide variety of other applications, including packaging. Because of its widespread use, there have been many studies evaluating this polymer. However, gaps still exist in our understanding of the hydrolytic degradation in extreme pH environments and its impact on physical and mechanical properties, especially...
Citation Formats
O. Y. Saiapina et al., “Conductometric enzyme biosensors based on natural zeolite clinoptilolite for urea determination,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 1490–1497, 2011, Accessed: 00, 2020. [Online]. Available: