Conductometric enzyme biosensors based on natural zeolite clinoptilolite for urea determination

Saiapina, O. Y.
Pyeshkova, V. M.
Soldatkin, O. O.
Melnik, V. G.
Akata Kurç, Burcu
Walcarius, A.
Dzyadevych, S. V.
Jaffrezic-Renault, N.
Highly sensitive conductometric urea biosensors were developed by exploiting the successful combination of ammonium-sieving and ion exchange properties of clinoptilolite, with a unique biorecognition capacity of urease. To optimize the performance of urea biosensors based on clinoptilolite, the dependences of their analytical signals on pH, buffer capacity and ionic strength of phosphate buffer solution (PBS) were studied. Optimum pH for urea biosensors was found within the range of pH 6.0-7.0. The dependences of biosensors responses on buffer capacity and ionic strength of PBS were of the same profile as those obtained for the urea biosensor which was not modified with clinoptilolite.


A novel urea conductometric biosensor based on zeolite immobilized urease
Kirdeciler, Salih Kaan; Soy, Esin; Öztürk, Seçkin; Kucherenko, Ivan; Soldatkin, Oleksandr; Dzyadevych, Sergei; Akata Kurç, Burcu (2011-09-15)
A new approach was developed for urea determination where a thin film of silicalite and zeolite Beta deposited onto gold electrodes of a conductometric biosensor was used to immobilize the enzyme. Biosensor responses, operational and storage stabilities were compared with results obtained from the standard membrane methods for the same measurements. For this purpose, different surface modification techniques, which are simply named as Zeolite Membrane Transducers (ZMTs) and Zeolite Coated Transducers (ZCTs)...
Application of enzyme/zeolite sensor for urea analysis in serum
Soldatkin, O. O.; Kucherenko, I. S.; Marchenko, S. V.; Kasap, B. Ozansoy; Akata Kurç, Burcu; Soldatkin, A. P.; Dzyadevych, S. V. (2014-09-01)
Urea biosensor based on zeolite-adsorbed urease was applied for analysis of blood serum samples. It should be noted, that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, high reproducibility and repeatability (RSD = 9% and 4%, respectively). The linear range of urea determination by using the biosensor was 0.003-0.75 mM, and the limit of urea detection was 3 mu M. The method of standard addition was used for analysi...
Direct evidence of advantage of using nanosized zeolite Beta for ISFET-based biosensor construction
Soy, Esin; Galioglu, Sezin; Soldatkin, Oleksandr O.; Dzyadevych, Sergei V.; Warzywoda, Juliusz; Sacco, Albert; Akata Kurç, Burcu (2013-05-01)
Analytical characteristics of urease- and butyrylcholinesterase (BuChE)-based ion sensitive field-effect transistor (ISFET) biosensors were investigated by the incorporation of zeolite Beta nanoparticles with varying Si/Al ratios. The results obtained by the zeolite-modified ISFET transducers suggested that the Si/Al ratio strongly influenced the biosensor performances due to the electrostatic interactions among enzyme, substrate, and zeolite surface as well as the nature of the enzymatic reaction. Using re...
Kucherenko, Ivan; Soldatkin, Oleksandr; Saiapina, Olga; Soy, Esin; Ozansoy, Berna; Kirdeciler, Salih Kaan; Öztürk, Seçkin; Akata Kurç, Burcu; Dzyadevych, Sergei (null; 2015-09-15)
In this work, the method of enzyme adsorption on different zeolites and mesoporous silica spheres (MSS) was investigated for the creation of conductometric biosensors. The conductometric transducers consisted of gold interdigitated electrodes were placed on the ceramic support. The transducers were modified with zeolites and MSS, and then the enzymes were adsorbed on the transducer surface. Different methods of zeolite attachment to the transducer surface were used; drop coating with heating to 200°C turned...
Hydrolytic Degradation of Polylactic Acid Fibers as a Function of pH and Exposure Time
Vaid, Radhika; Yıldırım, Erol; Pasquinelli, Melissa A.; King, Martin W. (2021-12-01)
Polylactic acid (PLA) is a widely used bioresorbable polymer in medical devices owing to its biocompatibility, bioresorbability, and biodegradability. It is also considered a sustainable solution for a wide variety of other applications, including packaging. Because of its widespread use, there have been many studies evaluating this polymer. However, gaps still exist in our understanding of the hydrolytic degradation in extreme pH environments and its impact on physical and mechanical properties, especially...
Citation Formats
O. Y. Saiapina et al., “Conductometric enzyme biosensors based on natural zeolite clinoptilolite for urea determination,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 1490–1497, 2011, Accessed: 00, 2020. [Online]. Available: