Hydrogels of agarose, and methacrylated gelatin and hyaluronic acid are more supportive for in vitro meniscus regeneration than three dimensional printed polycaprolactone scaffolds

Date

2019-02-01

Author

Bahcecioglu, Gokhan
Hasırcı, Nesrin
Bilgen, Bahar
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

125
views

0
downloads

In this study, porcine fibrochondrocyte-seeded agarose, methacrylated gelatin (GelMA), methacrylated hyaluronic acid (MeHA) and GelMA-MeHA blend hydrogels, and 3D printed PCL scaffolds were tested under dynamic compression for potential meniscal regeneration in vitro. Cell-carrying hydrogels produced higher levels of extracellular matrix (ECM) components after a 35-day incubation than the 3D printed PCL Cells on GelMA exhibited strong cell adhesion (evidenced with intense paxillin staining) and dendritic cell morphology, and produced an order of magnitude higher level of collagen (p < 0.05) than other materials. On the other hand, cells in agarose exhibited low cell adhesion and round cell morphology, and produced higher levels of glycosaminoglycans (GAGS) (p < 0.05) than other materials. A low level of ECM production and a high level of cell proliferation were observed on the 3D printed PCL. Dynamic compression at 10% strain enhanced GAG production in agarose (p < 0.05), and collagen production in GelMA. These results show that hydrogels have a higher potential for meniscal regeneration than the 3D printed PCL, and depending on the material used, fibrochondrocytes could be directed to proliferate or produce cartilaginous or fibrocartilaginous ECM. Agarose and MeHA could be used for the regeneration of the inner region of meniscus, while GelMA for the outer region.

Subject Keywords

Hydrogels, 3D printed PCL, Meniscal regeneration

URI

https://hdl.handle.net/11511/29949

Journal

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

DOI

https://doi.org/10.1016/j.ijbiomac.2018.09.065

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Hydroxyl-Terminated Poly(urethane acrylate) as a Soft Liner in Dental Applications: Synthesis and Characterization Keskin, Selda; Usanmaz, Ali (Wiley, 2010-07-05) Hydroxyl-terminated poly(urethane acrylate)s were synthesized for use in biomedical applications. Acrylate end capping via an interesterification reaction was successfully achieved with methacryloyl chloride addition to the hydroxyl ends of the polyurethane at low temperatures. 2,4-Toluene diisocyanate, 1,6-hexane diisocyanate, and methylene diphenyl diisocyanate were used as diisocyanates for urethane synthesis, and they were end-capped with methyl methacrylate and hydroxyethyl methacrylate. The nature of ...
Single-component layer-by-layer weak polyelectrolyte films and capsules: Loading and release of functional molecules Kozlovskaya, V. A.; Kharlampieva, E. P.; Erel Göktepe, İrem; Sukhishvili, S. A. (2009-06-01) Poly(carboxylic acid) hydrogel films and hollow capsules undergo reversible size changes in response to variations in pH and/or ionic strength. The films and capsules were obtained from hydrogenbonded poly-N-vinylpyrrolidone/poly(carboxylic acid) layer-by-layer films by chemical crosslinking of the polyacid, followed by pH-induced removal of poly-N-vinylpyrrolidone. Surface-attached hydrogel films present attractive matrices for reversible pH-stimulated loading and/or controlled release of large amounts of ...
Multi-crystalline silicon solar cells with metal-assisted nano-texturing using HNO3 as hole injection agent Es, Fırat; Baytemir, Gulsen; Kulakci, Mustafa; Turan, Raşit (2016-12-01) In this study, metal-assisted etching (MAE) with nitric acid (HNO3) as a hole injecting agent has been employed to texture multi-crystalline silicon wafers. It was previously proven that addition of HNO3 enabled control of surface texturing so as to form nano-cone shaped structures rather than nanowires. The process parameters optimized for optically efficient texturing have been applied to multi-crystalline wafers. Fabrication of p-type Al: BSF cells have been carried out on textured samples with thermal S...
Alkyl end group Modification of Benzotriazole and Thiophene Containing Conjugated Polymers Cevher, Şevki Can; Keleş, Duygu; Özdemir Hacıoğlu, Şerife; Toppare, Levent Kamil; Çırpan, Ali (null; 2016-07-21) This study focused on modifying the alkyl‐end‐group of benzotriazole and thiophene containing conjugated polymers. Introducing a moiety with struc‐turally rigid and tendency to enhance the π‐π stacking and also absorption in the high energetic region, influence the both electronic and optic properties of the conjugated polymers. Stille cross coupling chemical polymerization method was performed to synthesize the polymer contains: benzotriazole and bisthiophene. Characterizations of polymers were done in ter...
Rapid ionic liquid-supported nano-hybrid composite reinforced hollow-fiber electromembrane extraction followed by field-amplified sample injection-capillary electrophoresis: An effective approach for extraction and quantification of Imatinib mesylate in human plasma Forough, Mehrdad; Farhadi, Khalil; Eyshi, Ali; Molaei, Rahim; Khalili, Hedayat; Kouzegaran, Vahid Javan; Matin, Amir Abbas (2017-09-01) The focus of this study is development of a new, convenient, rapid and sensitive electromembrane extraction approach (based on an ionic liquid-supported MWCNTs/ZnO reinforced hollow fiber, for the first time) as an off-line sample clean-upipreconcentration method coupled with capillary electrophoresis (CE-UV) using field-amplified sample injection (FASI) for quantification of Imatinib mesylate in human plasma. The nano-hybrid sorbent, coated by 1-octyl-3-methylimidazolium bromide ionic liquid (E0M1m]Br) in ...

Citation Formats

G. Bahcecioglu, N. Hasırcı, B. Bilgen, and V. N. Hasırcı, “Hydrogels of agarose, and methacrylated gelatin and hyaluronic acid are more supportive for in vitro meniscus regeneration than three dimensional printed polycaprolactone scaffolds,” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, pp. 1152–1162, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29949.