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Fomes fomentarius and Tricholoma anatolicum (Agaricomycetes) Extracts Exhibit Significant Multiple Drug-Resistant Modulation Activity in Drug-Resistant Breast Cancer Cells
Date
2020-01-01
Author
DOĞAN, HASAN HÜSEYİN
Kars, Meltem Demirel
Ozdemir, Ozdem
Gündüz, Ufuk
Metadata
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Multiple drug resistance is one of the main problems that hinder successful cancer chemotherapy. Investigations on the development of effective chemotherapeutic agents and drug resistance inhibitors motivate studies on the effects of natural compounds on drug-resistant cancer cells. For this purpose, aqueous, methanol, and ethanol extracts of Fomes fomentarius and Tricholoma anatolicum were prepared. The extracts were evaluated to assess their anticancer and multiple drug resistance modulation activities. Cytotoxic effects of F. fomentarius and T. anatolicum extracts on paclitaxcl and vincristine resistant P-glycoprotein over-expressing MCF-7 cell lines were investigated by cytotoxicity test (XTT). P-glycoprotein reversing ability and MDR modulation effects of the extracts were determined by flow cytomeny through Rhodamine 123 exclusion assay. Furthermore, 11 phenolic compounds in the extracts were characterized by HPLC. As a result of the cytotoxicity assay, IC50 values of the extracts for MCF-7/Vinc were between 1.08 and 1.80 mg/mL, and IC50 values for MCF-7/Pac were found between 1.11 and 2.83 mg/mL. Strikingly, methanol extract of F. fomentalists and ethanol extract of T. anatolicum have potential value to become MDR reversing agents for drug-resistant breast cancer cells.
Subject Keywords
Antitumor effect
,
MDR reversal
,
Fomes fomentarius
,
Tricholoma anatolicum
,
Medicinal mushrooms
URI
https://hdl.handle.net/11511/30200
Journal
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
DOI
https://doi.org/10.1615/intjmedmushrooms.2020033174
Collections
Graduate School of Natural and Applied Sciences, Article
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H. H. DOĞAN, M. D. Kars, O. Ozdemir, and U. Gündüz, “Fomes fomentarius and Tricholoma anatolicum (Agaricomycetes) Extracts Exhibit Significant Multiple Drug-Resistant Modulation Activity in Drug-Resistant Breast Cancer Cells,”
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
, pp. 105–114, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30200.