A collagen-based corneal stroma substitute with micro-designed architecture

Date

2014-01-01

Author

Kilic, Cemile
Girotti, Alessandra
Rodriguez-Cabello, Carlos
Hasırcı, Vasıf Nejat

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A 3D corneal stroma substitute with micro-level patterns was constructed from a stack of 4 micro patterned collagen or collagen-elastin like recombinamer (Col:ELR) blend layers. The transparency of all of the films was quite high with the uncrosslinked (UXL) films and dehydrothermally treated (150 degrees C, 24 h) Col:ELR films yielding the best results. Human corneal keratocytes (HK) could be attached and proliferated equally well on the single films of Col and Col:ELR. However, for the multilayer constructs the proliferation was higher on Col than on Col:ELR. The cells were found to align along the patterns (microchannels with a 39 mu m groove depth, 8 mu m groove width, 3.3 mu m ridge width, and 54.7 degrees inclination angle) of the films, while no significant alignment was observed on the unpatterned films. The transparency of the seeded Col:ELR films was superior to the Col films over a 30 day incubation period and was quite close to that of a native human cornea. It was concluded that the Col and Col:ELR films and their 3D constructs have significant potential for use as corneal stroma substitutes.

Subject Keywords

Elastin-like polypeptide, Pigment epithelial-cells, Mechanical-properties, Tissue, Keratocytes, Scaffolds, Model, Reconstruction, Transparency, Transparency, Biomaterials

URI

https://hdl.handle.net/11511/30210

Journal

BIOMATERIALS SCIENCE

DOI

https://doi.org/10.1039/c3bm60194c

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

C. Kilic, A. Girotti, C. Rodriguez-Cabello, and V. N. Hasırcı, “A collagen-based corneal stroma substitute with micro-designed architecture,” BIOMATERIALS SCIENCE, pp. 318–329, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30210.