A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche

Date

2020-02-01

Author

Calamak, Semih
Ermiş Şen, Menekşe
Sun, Han
Islam, Saiful
Sikora, Michael
Nguyen, Michelle
Hasırcı, Vasıf Nejat
Steinmetz, Lars M.
Demirci, Utkan

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Cancer is a complex and heterogeneous disease, and cancer cells dynamically interact with the mechanical microenvironment such as hydrostatic pressure, fluid shear, and interstitial flow. These factors play an essential role in cell fate and circulating tumor cell heterogeneity, and can influence the cellular phenotype. In this study, a peristaltic continuous flow reactor is designed and applied to HCT-116 colorectal carcinoma cells to mimic the fluid dynamics of circulation. With this intervention, a CD44/CD24-cell subpopulation emerges, and 100 genes are significantly regulated. The expression of cells at 4 h in the flow reactor is very similar to TGF-ss treatment, which is an inducer of epithelial-mesenchymal transition. ATF3 and SERPINE1 are significantly upregulated in these groups, suggesting that the mesenchymal transition is induced through this signaling pathway. This flow reactor model is satisfactory on its own to reprogram colorectal cancer cells toward a more mesenchymal niche mimicking circulation of the blood.

Subject Keywords

Bioreactors, Circulating tumor cells, Epithelial to mesenchymal transition, Fluidics, Programming, Shear force

URI

https://hdl.handle.net/11511/30376

Journal

ADVANCED BIOSYSTEMS

DOI

https://doi.org/10.1002/adbi.201900139

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

S. Calamak et al., “A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche,” ADVANCED BIOSYSTEMS, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30376.