3D printed poly(epsilon-caprolactone) scaffolds modified with hydroxyapatite and poly(propylene fumarate) and their effects on the healing of rabbit femur defects

Date

2017-10-01

Author

Büyüksungur, Senem
Tanir, Tugba Endogan
Büyüksungur, Arda
Bektas, Ezgi Irem
Kose, Gamze Torun
Yücel, Deniz
Beyzadeoglu, Tahsin
Çetinkaya, Engin
Yenigün, Cagri
Tönük, Ergin
Hasırcı, Vasıf Nejat
Hasırcı, Nesrin

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A large variety of approaches have been used to treat large and irregular shaped bone defects with less than optimal success due to material or design issues. In recent years patient specific constructs prepared by additive manufacturing provided a solution to the need for shaping implants to fit irregular defects in the surgery theater. In this study, cylindrical disks of poly(e-caprolactone) (PCL) were printed by fused deposition modeling and modified with nanohydroxyapatite (HAp) and poly(propylene fumarate) (PPF) to create a mechanically strong implant with well-defined pore size and porosity, controllable surface hydrophilicity (with PPF) and osteoconductivity (with HAp). Cytotoxicity, irritation and inflammation tests demonstrated that the scaffolds were biocompatible. PCL/HAp and PCL/HAp/PPF scaffolds were implanted in the femurs of rabbits with and without seeding with rabbit Bone Marrow Stem Cells (BMSC) and examined after 4 and 8 weeks with micro-CT, mechanically and histologically. BMSC seeded PCL/HAp/PPF scaffolds showed improved tissue regeneration as determined by bone mineral density and micro-CT. Compressive and tension stiffness values (394 and 463 N mm(-1)) were significantly higher than those of the healthy rabbit femur (316 and 392 N mm(-1), respectively) after 8 weeks of implantation. These 3D implants have great potential for patient-specific bone defect treatments.

Subject Keywords

Cross-linking characteristics, Surface free-energy, Mechanical-properties, Osteogenic differentiation, Tricalcium phosphate, Plasma modification, Drug-release, Pmma films, Bone, Degradation

URI

https://hdl.handle.net/11511/30408

Journal

BIOMATERIALS SCIENCE

DOI

https://doi.org/10.1039/c7bm00514h

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

S. Büyüksungur et al., “3D printed poly(epsilon-caprolactone) scaffolds modified with hydroxyapatite and poly(propylene fumarate) and their effects on the healing of rabbit femur defects,” BIOMATERIALS SCIENCE, pp. 2144–2158, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30408.