A Mechanically Functional Collagen-Based Construct Designed as a Meniscus Substitute

2013-04-01
In this study, a novel 5-layered meniscus scaffold, constituted of 3 different collagen-based foams interspaced with two electrospun nano/microfibrous mats, was designed to mimic the mechanical and physical properties of the natural meniscus. The top layer was a collagen-chondroitin sulfate-hyaluronic acid (Coll-CS-HA) foam. The middle and bottom layers were collagen foams which had different porosities and compressive properties. The aligned mats in between the foams were a blend of collagen and poly(L-lactic acid-co-glycolic acid). For the 5-layered construct, the compressive, tensile and shear moduli were 445 KPa, 3 MPa and 194 KPa, respectively. In dry state, the construct possessed higher compressive and shear properties than the natural tissue but the tensile properties were much lower. The mechanical properties are expected to be improved when the cells are seeded on the scaffold by their secretion of collagen and GAG.
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING

Suggestions

A multilayer tissue engineered meniscus substitute
Halili, Albana Ndreu; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2014-04-01)
Various methods have been tried to treat the main meniscus problem, meniscal tears, for which we believe tissue engineering could be a viable solution. In this study, a three dimensional, collagen-based meniscus substitute was prepared by tissue engineering using human fibrochondrocytes and a collagen based-scaffold. This construct was made with 3 different collagen-based foams interspaced with two electrospun nano/microfibrous mats. The top layer was made of collagen type I-chondroitin sulfate-hyaluronic a...
Construction and in vitro testing of a multilayered, tissue-engineered meniscus
Bahcecioglu, Gokhan; Buyuksungur, Arda; Kızıltay, Aysel; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2014-05-01)
A novel three-dimensional construct was designed to serve as a substitute for the natural meniscus tissue, and tested in vitro. The design consisted of mats of aligned collagen micro/nanofibers, entrapped within a macroporous poly(l-lactic acid)/poly(lactic acid-co-glycolic acid) foam coated with Ca+2-cross-linked alginic acid. Fibrochondrocytes derived from human meniscus were tested in vitro to study cell attachment and proliferation. After a 21-day culture, the cells populating the constructs were shown ...
A CRITICAL REEVALUATION OF STRESSES GENERATED DURING VERTICAL AND LATERAL CONDENSATION OF GUTTA-PERCHA IN THE ROOT-CANAL
TELLI, C; GULKAN, P; Günel, Mehmet Halis (Wiley, 1994-02-01)
The finite element method was used to calculate the stresses in a maxillary canine tooth produced during lateral and vertical condensation. Description of the biological domain to be analyzed, and conversion of the manual operations of the endodontist to realistic load representation are intricate problems which must be overcome before stresses are recovered and plotted. The stresses in dentin are in general of comparable magnitudes during lateral or vertical condensation, but these magnitudes generally rem...
A PHASE FIELD APPROACH TO MODEL FRACTURE OF ARTERIAL WALLS
GÜLTEKİN, Osman; Dal, Hüsnü; HOLZAPFEL, Gerhard A (2016-06-10)
This study uses a recently developed phase-field approach to model fracture of arterial walls with an emphasis on aortic tissues. We start by deriving the regularized crack surface to overcome complexities inherent in sharp crack discontinuities, thereby relaxing the acute crack surface topology into a diffusive one. In fact, the regularized crack surface possesses the property of Gamma-Convergence, i.e. the sharp crack topology is restored with a vanishing length-scale parameter. Next, we deal with the con...
A surface crack in a graded coating subjected to sliding frictional contact
Dağ, Serkan; GÜLER, MEHMET ALİ; Gulgec, Mufit (Elsevier BV, 2012-01-01)
This article presents an analytical method developed to conduct fracture analysis of functionally graded coatings that are under the effect of contact stresses. The geometric model considered comprises a functionally graded coating, which possesses a surface crack and is perfectly bonded to a homogeneous half-plane. A two-step solution procedure is put forward in which the contact and crack problems are treated separately. First, the graded coating is assumed to be in sliding frictional contact with a rigid...
Citation Formats
A. N. Halili, N. Hasırcı, and V. N. Hasırcı, “A Mechanically Functional Collagen-Based Construct Designed as a Meniscus Substitute,” JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, pp. 173–184, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30513.