Changes in apoptosis-related gene expression and cytokine release in breast cancer cells treated with CpG-loaded magnetic PAMAM nanoparticles

Date

2016-12-30

Author

Pourianazar, Negar Taghavi
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

57
views

0
downloads

CpG-oligodeoxynucleotide (CpG-ODN) can function as an immune adjuvant. Previously, we showed that stimulation of breast cancer cells with CpG-ODN conjugated with PAMAM dendrimer-coated magnetic nanoparticles (DcMNPs) has induced apoptosis. The aim of the current study was to evaluate the expression levels of some apoptosis-regulating genes in several human breast cancer cells treated with CpG/DcMNPs. Treated MDA-MB231 cells showed an increase in Noxa and Bax gene expression levels, whereas the expression level of Survivin decreased. Similarly, Noxa gene was overexpressed in treated MCF7 cells. In treated SKBR3 cells, a decline in the c-Flip mRNA level was determined. Furthermore, release of cytokines, IL-6, IL-10, and TNF-alpha, was determined in cell culture supernatants. CpG/DcMNP treatment leads to an increase in the release of IL-6 in MDA-MB231 and SKBR3 cells, whereas release of IL-10 and TNF-alpha did not change significantly. It is indicated that CpG-ODN may show its cytotoxic effect by regulating the expression of apoptosis-related genes and the release of cytokine in breast cancer cells.

Subject Keywords

CpG-ODN, Apoptosis-related genes, Cytokine release, Dendrimeric nanoparticles, Magnetic nanoparticles

URI

https://hdl.handle.net/11511/30605

Journal

INTERNATIONAL JOURNAL OF PHARMACEUTICS

DOI

https://doi.org/10.1016/j.ijpharm.2016.10.007

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Regulation of Cytoskeletal Dynamics during T Cell Activation by Substrate Stiffness Özçelikkale, Altuğ; Upadhyaya, Arpita (2018-03-07) T lymphocytes are an integral part of the adaptive immune response. The detection of infectious agents critically depends on the interaction of T cells with antigen presenting cells, which have varying mechanical stiffness and complex topological features. It has been recently recognized that T cell activation is regulated both by stiffness of the antigen presenting surface and by cytoskeletal forces which partially arise from actomyosin contractility. However, the relationship between stiffness and ...
Cytotoxic T Lymphocyte Activation Signals Modulate Cytoskeletal Dynamics and Mechanical Force Generation Pathni, Aashli; Özçelikkale, Altuğ; Rey-Suarez, Ivan; Li, Lei; Davis, Scott; Rogers, Nate; Xiao, Zhengguo; Upadhyaya, Arpita (2022-03-01) Cytotoxic T lymphocytes (CTLs) play an integral role in the adaptive immune response by killing infected cells. Antigen presenting cells (APCs), such as dendritic cells, present pathogenic peptides to the T cell receptor on the CTL surface and co-stimulatory signals required for complete activation. Activated CTLs secrete lytic granules containing enzymes that trigger target cell death at the CTL-target contact, also known as the immune synapse (IS). The actin and microtubule cytoskeletons are instrumental ...
Determination of immunomodulatory effects of SARS-COV-2 structural, non-structural, and accessory proteins on macrophage-like cells in the context of type I IFN antagonism and inflammasome activation Aydın, Yağmur; Gürsel, Mayda; Department of Biology (2022-2-02) Infection with SARS-CoV-2 inhibits early type I interferon response but activates inflammasome signaling and pro-inflammatory cytokine production. The virus accomplishes these opposing effects by manipulating the host cell immunity through a multitude of encoded viral proteins of structural, non-structural, or accessory origin. In this thesis, to investigate the immunomodulatory effects of SARS-CoV-2 encoded proteins on type I IFN antagonism and inflammasome activation, five SARS-CoV-2 structural, nonstruct...
Expression of Multidrug Resistance 1, Lung Resistance Protein and Breast Cancer Resistance Protein Genes in Chronic Leukemias Iseri, Ozlem D.; Kars, Meltem D.; Mutlu, Pelin; Avcu, Ferit; Ural, Ali U.; Gündüz, Ufuk (2011-01-01) One of the major problems in treatment of leukemias is multidrug resistance, which is already present at diagnosis or develops after chemotherapy. The gene expression levels of multidrug resistance resistance 1 (MDR1), breast cancer resistance protein (BCRP) and lung resistance-resistance protein (LRP) were evaluated in blood samples of 20 CLL and 24 CML patients using RT-PCR. MDR1, BCRP and LRP expression levels were detected in 65%, 20% and 45% of CLL patients, and in 54%, 37% and 25% of CML patients, res...
Impaired toll like receptor-7 and 9 induced immune activation in chronic spinal cord injured patients contributes to immune dysfunction Gucluler, Gozde; Adiguzel, Emre; Gungor, Bilgi; Kahraman, Tamer; Gürsel, Mayda; Yilmaz, Bilge; GÜRSEL, İHSAN (Public Library of Science (PLoS), 2017-02-07) Reduced immune activation or immunosuppression is seen in patients withneurological diseases. Urinary and respiratory infections mainly manifested as septicemia and pneumonia are the most frequent complications following spinal cord injuries and they account for the majority of deaths. The underlying reason of these losses is believed to arise due to impaired immune responses to pathogens. Here, we hypothesized that susceptibility to infections of chronic spinal cord injured (SCI) patients might be due to i...

Citation Formats

N. T. Pourianazar and U. Gündüz, “Changes in apoptosis-related gene expression and cytokine release in breast cancer cells treated with CpG-loaded magnetic PAMAM nanoparticles,” INTERNATIONAL JOURNAL OF PHARMACEUTICS, pp. 11–19, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30605.