Topographically induced self-deformation of the nuclei of cells: dependence on cell type and proposed mechanisms

Date

2010-03-01

Author

Davidson, Patricia M.
Fromigue, Olivia
Marie, Pierre J.
Hasırcı, Vasıf Nejat
Reiter, Guenter
Anselme, Karine

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Osteosarcoma-derived cell lines (SaOs-2, MG63) have recently been shown to deform their nucleus considerably in response to surface topography. Such a deformation had not been described previously. Here we present results on additional cell lines, including cancerous (OHS4, U2OS), immortalized (F/STRO-1(+)A and FHSO6) and healthy cells (HOP). The cancerous cells were found to deform extensively, the immortalized cells showed small deformations, whereas the healthy cells showed deformation only at short incubation times. These results suggest a strong link between the malignant transformation of cells and the state of the cytoskeletal network. We propose mechanisms to explain the deformation in which the cytoskeleton either pushes down on the nucleus during spreading or pulls it down upon adhesion to the pillars.

Subject Keywords

Marrow stromal cells, Mechanotransduction, Transformation, Deformability, Fibroblasts, Expression, Proteins, Matrix, Actin, Force

URI

https://hdl.handle.net/11511/31240

Journal

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

DOI

https://doi.org/10.1007/s10856-009-3950-7

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

P. M. Davidson, O. Fromigue, P. J. Marie, V. N. Hasırcı, G. Reiter, and K. Anselme, “Topographically induced self-deformation of the nuclei of cells: dependence on cell type and proposed mechanisms,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, pp. 939–946, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31240.