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Multifunctional periodontal membrane for treatment and regeneration purposes
Date
2020-03-01
Author
Isik, Gulhan
Hasırcı, Nesrin
Tezcaner, Ayşen
Kızıltay, Aysel
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Periodontitis is a chronic inflammatory disease that causes gum tissue degeneration and alveolar bone and tooth loss. The aim of this study is to develop a multifunctional matrix for the treatment of periodontitis and enhancement of regeneration of the periodontal tissue. The matrix was prepared from vitamin E containing hydrogel made of alginate and gelatin, and doxycycline HCl containing methoxy poly(ethylene glycol)-block-polycaprolactone micelles. Methoxy poly(ethylene glycol)-block-polycaprolactone was synthesized with ring-opening polymerization technique and characterized by proton nuclear magnetic resonance (H-1 NMR), Fourier-transform infrared spectroscopy, differential scanning calorimetry, and gel permeation chromatography. Micelles were characterized by measuring zeta potential, hydrodynamic diameter, drug encapsulation efficiency, drug loading capacity, and in vitro drug-release kinetics. Micelles were obtained with an average size of 164 nm and drug loading amount of 5.8%. The activity of doxycycline HCl-loaded micelles and vitamin E containing hydrogels was determined against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) with disk diffusion method. Bio-efficacy of micelle-loaded alginate-gelatin hydrogels were tested in vitro using L929 fibroblasts and dental pulp stem cells. Doxycycline HCl-loaded micelles and vitamin E containing hydrogels showed a sustained release and exhibited inhibition zone against E. coli and S. aureus. Hydrogels with vitamin E and doxycycline HCl-loaded micelles promoted osteogenic differentiation of dental pulp stem cells. Results suggest that alginate-gelatin hydrogels containing doxycycline HCl-loaded micelles and vitamin E can be good candidates for the treatment of periodontitis and tissue regeneration.
Subject Keywords
Methoxy poly(ethylene glycol)-block-polycaprolactone micelle
,
Alginate
,
Gelatin
,
Vitamin E
,
Doxycycline
,
Periodontitis
URI
https://hdl.handle.net/11511/31271
Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
DOI
https://doi.org/10.1177/0883911520911659
Collections
Graduate School of Natural and Applied Sciences, Article
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G. Isik, N. Hasırcı, A. Tezcaner, and A. Kızıltay, “Multifunctional periodontal membrane for treatment and regeneration purposes,”
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
, pp. 117–138, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31271.