Multifunctional periodontal membrane for treatment and regeneration purposes

2020-03-01
Periodontitis is a chronic inflammatory disease that causes gum tissue degeneration and alveolar bone and tooth loss. The aim of this study is to develop a multifunctional matrix for the treatment of periodontitis and enhancement of regeneration of the periodontal tissue. The matrix was prepared from vitamin E containing hydrogel made of alginate and gelatin, and doxycycline HCl containing methoxy poly(ethylene glycol)-block-polycaprolactone micelles. Methoxy poly(ethylene glycol)-block-polycaprolactone was synthesized with ring-opening polymerization technique and characterized by proton nuclear magnetic resonance (H-1 NMR), Fourier-transform infrared spectroscopy, differential scanning calorimetry, and gel permeation chromatography. Micelles were characterized by measuring zeta potential, hydrodynamic diameter, drug encapsulation efficiency, drug loading capacity, and in vitro drug-release kinetics. Micelles were obtained with an average size of 164 nm and drug loading amount of 5.8%. The activity of doxycycline HCl-loaded micelles and vitamin E containing hydrogels was determined against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) with disk diffusion method. Bio-efficacy of micelle-loaded alginate-gelatin hydrogels were tested in vitro using L929 fibroblasts and dental pulp stem cells. Doxycycline HCl-loaded micelles and vitamin E containing hydrogels showed a sustained release and exhibited inhibition zone against E. coli and S. aureus. Hydrogels with vitamin E and doxycycline HCl-loaded micelles promoted osteogenic differentiation of dental pulp stem cells. Results suggest that alginate-gelatin hydrogels containing doxycycline HCl-loaded micelles and vitamin E can be good candidates for the treatment of periodontitis and tissue regeneration.

Citation Formats
G. Isik, N. Hasırcı, A. Tezcaner, and A. Kızıltay, “Multifunctional periodontal membrane for treatment and regeneration purposes,” JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, vol. 35, no. 2, pp. 117–138, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31271.