A multilayer tissue engineered meniscus substitute

Various methods have been tried to treat the main meniscus problem, meniscal tears, for which we believe tissue engineering could be a viable solution. In this study, a three dimensional, collagen-based meniscus substitute was prepared by tissue engineering using human fibrochondrocytes and a collagen based-scaffold. This construct was made with 3 different collagen-based foams interspaced with two electrospun nano/microfibrous mats. The top layer was made of collagen type I-chondroitin sulfate-hyaluronic acid (Coll-CS-HA), and the middle and the bottom layers were made of only collagen type I with different porosities and thus with different mechanical properties. The mats of aligned fibers were a blend of collagen type I and poly(l-lactic acid-co-glycolic acid) (PLGA). After seeding with human fibrochondrocytes, cell attachment, proliferation, and production of extracellular matrix and glucoseaminoglycan were studied. Cell seeding had a positive effect on the compressive properties of foams and the 3D construct. The 3D construct with all its 5 layers had better mechanical properties than the individual foams.


A Mechanically Functional Collagen-Based Construct Designed as a Meniscus Substitute
Halili, Albana Ndreu; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2013-04-01)
In this study, a novel 5-layered meniscus scaffold, constituted of 3 different collagen-based foams interspaced with two electrospun nano/microfibrous mats, was designed to mimic the mechanical and physical properties of the natural meniscus. The top layer was a collagen-chondroitin sulfate-hyaluronic acid (Coll-CS-HA) foam. The middle and bottom layers were collagen foams which had different porosities and compressive properties. The aligned mats in between the foams were a blend of collagen and poly(L-lac...
A novel urea conductometric biosensor based on zeolite immobilized urease
Kirdeciler, Salih Kaan; Soy, Esin; Öztürk, Seçkin; Kucherenko, Ivan; Soldatkin, Oleksandr; Dzyadevych, Sergei; Akata Kurç, Burcu (2011-09-15)
A new approach was developed for urea determination where a thin film of silicalite and zeolite Beta deposited onto gold electrodes of a conductometric biosensor was used to immobilize the enzyme. Biosensor responses, operational and storage stabilities were compared with results obtained from the standard membrane methods for the same measurements. For this purpose, different surface modification techniques, which are simply named as Zeolite Membrane Transducers (ZMTs) and Zeolite Coated Transducers (ZCTs)...
A novel blue-emitting phosphor: BaAl(2)B(2)O(7): Pb(2+)
Pekgozlu, I.; Seyyidoglu, S.; TAŞCIOĞLU, SÜLİN (Elsevier BV, 2008-09-01)
Pb(2+) doped BaAl(2)B(2)O(7) materials were prepared by a solution combustion synthesis. The phase of the synthesized materials was determined using the powder X-ray diffraction. The photoluminescent properties of Pb(2+) doped BaAl(2)B(2)O(7) materials were investigated using spectrofluorometer at room temperature. The emission and excitation bands of BaAl(2)B(2)O(7): Pb(2+) were observed at 423 and 266 nm, respectively. The dependence of the emission intensity on the Pb(2+) concentration for BaAl(2)B(2)O(7...
A new processable and fluorescent polydithienylpyrrole electrochrome with pyrene appendages
TİRKEŞ, SEHA; Mersini, Jetmire; Oztas, Zahide; Algi, Melek Pamuk; Algi, Fatih; CİHANER, ATİLLA (2013-02-15)
A new hybrid compound, namely 1-(pyren-3-yl)-2,5-di(thiophen-2-yl)-1H-pyrrole (SNS-P), was polymerized via both chemical and electrochemical methods. Chemically obtained soluble polydithienylpyrrole (c-PSNS-P) bearing pyrene appendages is a homogeneous and uniform polymer with a number averaged molecular weight of 15,200 g/mol. The polymer exhibits both multi-electrochromic and fluorescent properties. Upon oxidation, the color of electrochemically obtained polymer (e-PSNS-P) changes from yellowish orange to...
Construction and in vitro testing of a multilayered, tissue-engineered meniscus
Bahcecioglu, Gokhan; Buyuksungur, Arda; Kızıltay, Aysel; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2014-05-01)
A novel three-dimensional construct was designed to serve as a substitute for the natural meniscus tissue, and tested in vitro. The design consisted of mats of aligned collagen micro/nanofibers, entrapped within a macroporous poly(l-lactic acid)/poly(lactic acid-co-glycolic acid) foam coated with Ca+2-cross-linked alginic acid. Fibrochondrocytes derived from human meniscus were tested in vitro to study cell attachment and proliferation. After a 21-day culture, the cells populating the constructs were shown ...
Citation Formats
A. N. Halili, N. Hasırcı, and V. N. Hasırcı, “A multilayer tissue engineered meniscus substitute,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, pp. 1195–1209, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31623.