A multilayer tissue engineered meniscus substitute

2014-04-01
Various methods have been tried to treat the main meniscus problem, meniscal tears, for which we believe tissue engineering could be a viable solution. In this study, a three dimensional, collagen-based meniscus substitute was prepared by tissue engineering using human fibrochondrocytes and a collagen based-scaffold. This construct was made with 3 different collagen-based foams interspaced with two electrospun nano/microfibrous mats. The top layer was made of collagen type I-chondroitin sulfate-hyaluronic acid (Coll-CS-HA), and the middle and the bottom layers were made of only collagen type I with different porosities and thus with different mechanical properties. The mats of aligned fibers were a blend of collagen type I and poly(l-lactic acid-co-glycolic acid) (PLGA). After seeding with human fibrochondrocytes, cell attachment, proliferation, and production of extracellular matrix and glucoseaminoglycan were studied. Cell seeding had a positive effect on the compressive properties of foams and the 3D construct. The 3D construct with all its 5 layers had better mechanical properties than the individual foams.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

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Citation Formats
A. N. Halili, N. Hasırcı, and V. N. Hasırcı, “A multilayer tissue engineered meniscus substitute,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, pp. 1195–1209, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31623.