Transcriptome analysis of Rhodobacter capsulatus grown on different nitrogen sources

Date

2019-07-01

Author

Erkal, Nilufer Afsar
Eser, Muazzez Gurgan
Ozgur, Ebru
Gündüz, Ufuk
EROĞLU, İNCİ
Yucel, Meral

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This study investigated the effect of different nitrogen sources, namely, ammonium chloride and glutamate, on photoheterotrophic metabolism of Rhodobacter capsulatus grown on acetate as the carbon source. Genes that were significantly differentially expressed according to Affymetrix microarray data were categorized into Clusters of Orthologous Groups functional categories and those in acetate assimilation, hydrogen production, and photosynthetic electron transport pathways were analyzed in detail. Genes related to hydrogen production metabolism were significantly downregulated in cultures grown on ammonium chloride when compared to those grown on glutamate. In contrast, photosynthetic electron transport and acetate assimilation pathway genes were upregulated. In detail, aceA encoding isocitrate lyase, a unique enzyme of the glyoxylate cycle and ccrA encoding the rate limiting crotonyl-CoA carboxylase/reductase enzyme of ethylmalonyl-coA pathway were significantly upregulated. Our findings indicate for the first time that R. capsulatus can operate both glyoxylate and ethylmalonyl-coA cycles for acetate assimilation.

Subject Keywords

Photoheterotrophic growth, Rhodobacter capsulatus, Purple non-sulfur bacteria, Microarray, Hydrogen metabolism, Acetate metabolism

URI

https://hdl.handle.net/11511/32208

Journal

ARCHIVES OF MICROBIOLOGY

DOI

https://doi.org/10.1007/s00203-019-01635-x

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

N. A. Erkal, M. G. Eser, E. Ozgur, U. Gündüz, İ. EROĞLU, and M. Yucel, “Transcriptome analysis of Rhodobacter capsulatus grown on different nitrogen sources,” ARCHIVES OF MICROBIOLOGY, pp. 661–671, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32208.