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Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor

2016-03-31
Marchenko, Svitlana V.
Soldatkin, Oleksandr O.
Kasap, Berna Ozansoy
Akata Kurç, Burcu
Soldatkin, Alexei P.
Dzyadevych, Sergei V.
In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehycie vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two-to threefold increased sensitivity to creatinine, three-to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 mu m, etc.).