Urease-based ISFET biosensor for arginine determination

Date

2014-04-01

Author

Sheliakina, M.
Arkhypova, V.
Soldatkin, O.
Saiapina, O.
Akata Kurç, Burcu
Dzyadevych, S.

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In this work a novel biosensor for arginine determination based on the urease inhibition effect has been proposed. Ion-selective field effect transistors were used as transducers. Urease immobilized in glutaraldehyde vapor served as a biorecognition element of the biosensor. Significant part of the work was aimed at proving the urease inhibition by arginine. Optimal concentration of urea for arginine determination was chosen. Detection limit for arginine was 0.05 mM. The biosensor selectivity towards different amino acids was studied. The results of quantitative determination of L-arginine in the real sample (a drinkable solution "Arginine Veyron") were in good agreement with the producer's data (a relative error was 5.2%). The biosensor showed a good reproducibility of arginine determination.

Subject Keywords

Ion-selective field effect transistors, Arginine, Urease, Biosensor, Inhibition

URI

https://hdl.handle.net/11511/32328

Journal

TALANTA

DOI

https://doi.org/10.1016/j.talanta.2013.12.042

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

M. Sheliakina, V. Arkhypova, O. Soldatkin, O. Saiapina, B. Akata Kurç, and S. Dzyadevych, “Urease-based ISFET biosensor for arginine determination,” TALANTA, pp. 18–23, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32328.