Single-stage photofermentative biohydrogen production from sugar beet molasses by different purple non-sulfur bacteria

Sagir, Emrah
Ozgur, Ebru
Gündüz, Ufuk
Eroglu, Inci
Yücel, Ayşe Meral
Biohydrogen production via fermentative routes offers considerable advantages in waste recycling and sustainable energy production. This can be realized by single-stage dark or photofermentative processes, or by a two-stage integrated process; the latter offering the higher production yields due to complete conversion of sugar substrates into H-2 and CO2. However, problems arising from the integration of these two processes limit its scale-up and implementation. Hence, high efficiency one-step fermentative biohydrogen production processes from sugar-rich wastes are preferable. In this study, different strains of purple non-sulfur bacteria were investigated for their biohydrogen production capacity on pure sucrose and sugar beet molasses, and the feasibility of single-stage photofermentative biohydrogen production was evaluated. A single-stage photofermentation process was carried out using four different strains of purple non-sulfur bacteria (Rhodobacter capsulatus DSM 1710, R. capsulatus YO3, Rhodobacter sphaeroides O.U.001, and Rhodopseudomonas palustris DSM 127) on different initial sucrose concentrations. The highest hydrogen yield obtained was 10.5 mol H-2/mol of sucrose and the maximum hydrogen productivity was 0.78 mmol/L h by Rp. palustris on 5 mM sucrose. A hydrogen yield of 19 mol H-2/mol sucrose, which represents 79% of theoretical yield, and a maximum hydrogen productivity of 0.55 mmol/L h were obtained by Rp. palustris from sugar beet molasses. The yield was comparable to those values obtained in two-stage processes. The present study demonstrates that single-stage photofermentation using purple non-sulfur bacteria on sucrose-based wastes is promising.


Hydrogen production by hup(-) mutant and wild-type strains of Rhodobacter capsulatus from dark fermentation effluent of sugar beet thick juice in batch and continuous photobioreactors
Uyar, Basar; Gurgan, Muazzez; Ozgur, Ebru; Gündüz, Ufuk; Yücel, Ayşe Meral; Eroglu, Inci (2015-10-01)
Photofermentative production of hydrogen is a promising and sustainable process; however, it should be coupled to dark fermentation to become cost effective. In order to integrate dark fermentation and photofermentation, the suitability of dark fermenter effluents for the photofermentative hydrogen production must be demonstrated. In this study, thermophilic dark fermenter effluent (DFE) of sugar beet thick juice was used as a substrate in photofermentation process to compare wild-type and uptake hydrogenas...
Biohydrogen production from beet molasses by sequential dark and photofermentation
Ozgur, Ebru; Mars, Astrid E.; Peksel, Beguem; Louwerse, Annemarie; Yucel, Meral; Gündüz, Ufuk; Claassen, Pieternel A. M.; Eroglu, Inci (2010-01-01)
Biological hydrogen production using renewable resources is a promising possibility to generate hydrogen in a sustainable way. In this study, a sequential dark and photofermentation has been employed for biohydrogen production using sugar beet molasses as a feedstock. An extreme thermophile Caldicellulosiruptor saccharolyticus was used for the dark fermentation, and several photosynthetic bacteria (Rhodobacter capsulatus wild type, R. capsulatus hup(-) mutant, and Rhodopseudomonas palustris) were used for t...
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To maintain the sustainability of cement and concrete production, there is a trend to use wastes in their production. Soda waste, generated by soda ash production process, and beer waste, generated by beer filtration process, are two locally produced wastes in Turkey and many other countries. The nature of these wastes, mostly their fineness, makes them possible to be used in concrete production, especially as a viscosity modifying agent in the self consolidating type of concrete. In this study, the additio...
Citation Formats
E. Sagir, E. Ozgur, U. Gündüz, I. Eroglu, and A. M. Yücel, “Single-stage photofermentative biohydrogen production from sugar beet molasses by different purple non-sulfur bacteria,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, pp. 1589–1601, 2017, Accessed: 00, 2020. [Online]. Available: