Single-stage photofermentative biohydrogen production from sugar beet molasses by different purple non-sulfur bacteria

Date

2017-11-01

Author

Sagir, Emrah
Ozgur, Ebru
Gündüz, Ufuk
Eroglu, Inci
Yücel, Ayşe Meral

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

156
views

0
downloads

Biohydrogen production via fermentative routes offers considerable advantages in waste recycling and sustainable energy production. This can be realized by single-stage dark or photofermentative processes, or by a two-stage integrated process; the latter offering the higher production yields due to complete conversion of sugar substrates into H-2 and CO2. However, problems arising from the integration of these two processes limit its scale-up and implementation. Hence, high efficiency one-step fermentative biohydrogen production processes from sugar-rich wastes are preferable. In this study, different strains of purple non-sulfur bacteria were investigated for their biohydrogen production capacity on pure sucrose and sugar beet molasses, and the feasibility of single-stage photofermentative biohydrogen production was evaluated. A single-stage photofermentation process was carried out using four different strains of purple non-sulfur bacteria (Rhodobacter capsulatus DSM 1710, R. capsulatus YO3, Rhodobacter sphaeroides O.U.001, and Rhodopseudomonas palustris DSM 127) on different initial sucrose concentrations. The highest hydrogen yield obtained was 10.5 mol H-2/mol of sucrose and the maximum hydrogen productivity was 0.78 mmol/L h by Rp. palustris on 5 mM sucrose. A hydrogen yield of 19 mol H-2/mol sucrose, which represents 79% of theoretical yield, and a maximum hydrogen productivity of 0.55 mmol/L h were obtained by Rp. palustris from sugar beet molasses. The yield was comparable to those values obtained in two-stage processes. The present study demonstrates that single-stage photofermentation using purple non-sulfur bacteria on sucrose-based wastes is promising.

Subject Keywords

Single-stage photofermentation, Sugar beet molasses, Sucrose, Purple non-sulfur bacterium, Biohydrogen

URI

https://hdl.handle.net/11511/32348

Journal

BIOPROCESS AND BIOSYSTEMS ENGINEERING

DOI

https://doi.org/10.1007/s00449-017-1815-x

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Ethanol steam reforming with zirconia based catalysts Arslan, Arzu; Doğu, Timur; Department of Chemical Engineering (2014) Production of hydrogen, which has been considered as an environmentally clean ideal energy carrier, from abundant energy resources cleanly and renewably is essential to support sustainable energy development. Hydrogen production from bio-ethanol by steam reforming process is a promising approach, since bio-ethanol is the most available bio-fuel in the world and steam reforming of ethanol yields formation of 6 moles of hydrogen per mole of ethanol. Support material used for nickel based catalysts plays a cru...
Hydrogen production by hup(-) mutant and wild-type strains of Rhodobacter capsulatus from dark fermentation effluent of sugar beet thick juice in batch and continuous photobioreactors Uyar, Basar; Gurgan, Muazzez; Ozgur, Ebru; Gündüz, Ufuk; Yücel, Ayşe Meral; Eroglu, Inci (2015-10-01) Photofermentative production of hydrogen is a promising and sustainable process; however, it should be coupled to dark fermentation to become cost effective. In order to integrate dark fermentation and photofermentation, the suitability of dark fermenter effluents for the photofermentative hydrogen production must be demonstrated. In this study, thermophilic dark fermenter effluent (DFE) of sugar beet thick juice was used as a substrate in photofermentation process to compare wild-type and uptake hydrogenas...
Biohydrogen production from beet molasses by sequential dark and photofermentation Ozgur, Ebru; Mars, Astrid E.; Peksel, Beguem; Louwerse, Annemarie; Yucel, Meral; Gündüz, Ufuk; Claassen, Pieternel A. M.; Eroglu, Inci (2010-01-01) Biological hydrogen production using renewable resources is a promising possibility to generate hydrogen in a sustainable way. In this study, a sequential dark and photofermentation has been employed for biohydrogen production using sugar beet molasses as a feedstock. An extreme thermophile Caldicellulosiruptor saccharolyticus was used for the dark fermentation, and several photosynthetic bacteria (Rhodobacter capsulatus wild type, R. capsulatus hup(-) mutant, and Rhodopseudomonas palustris) were used for t...
Photofermentative hydrogen production from molasses: Scale-up and outdoor operation at low carbon-to-nitrogen ratio Savasturk, Dilan; Kayahan, Emine; Koku, Harun (2018-06-28) Photofermentative hydrogen production was carried out under outdoor conditions with a Rhodobacter capsulatus strain on molasses, a renewable and sustainable feedstock. An existing photobioreactor design was scaled-up from 9 L to 20 L. The decreased carbon-to nitrogen (C/N) ratio of 13.0, compared to our previous work, accelerated growth and resulted in a reduced lag period for hydrogen production as well as higher productivities in the exponential phase. However, the low C/N ratio also promoted a high optic...
Dual-band perfect metamaterial absorber for solar cell applications Rufangura, Patrick; Sabah, Cumali (2015-10-01) The efficiency of solar photovoltaic (PV) cells has been one of the major problems impeding its global adoption as one of the sustainable substitutes to fossil fuel based technologies. Metamaterial (MTM) based solar cells offer an opportunity towards increasing the system efficiency by enhancing the total absorbed solar radiation incident on this device. In this study, a nanostructure-based MTM perfect absorber has been designed and simulated. By adjusting geometrical parameters and MTM structure properties...

Citation Formats

E. Sagir, E. Ozgur, U. Gündüz, I. Eroglu, and A. M. Yücel, “Single-stage photofermentative biohydrogen production from sugar beet molasses by different purple non-sulfur bacteria,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, pp. 1589–1601, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32348.