Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF

Onalan, G
Selam, B
Baran, Y
Cincik, M
Onalan, R
Gündüz, Ufuk
Ural, AU
Pabuccu, R
BACKGROUND: There are limited data about the levels of soluble apoptotic factors and their modulation with therapeutic regimens in IVF cycles. The aim of the current study was to determine follicular fluid, and serum levels of soluble Fas (sFas) and soluble Fas ligand (sFasL) in PCOS patients undergoing IVF/ICSI cycles; also to investigate the effects of metformin on these factors and on apoptosis of luteinized granulosa cells. METHODS: We investigated the serum and follicular fluid levels of sFas and sFasL in patients with PCOS (n=28) and compared them with those of the patients with infertility due to male factor (n=12) undergoing IVF cycles. Effects of metformin therapy on these parameters and apoptosis of luteinized granulosa cells were also investigated among the patients with PCOS. RESULTS: Serum levels of sFas were significantly lower in the PCOS group compared to those in women with infertility due to male factor. Metformin therapy in PCOS patients preceding IVF cycles increased serum levels of sFas and decreased follicular fluid levels of sFasL compared to those on placebo. Follicular fluid from PCOS patients demonstrated luteinized granulosa cell DNA fragmentation in agarose gel, whereas a similar pattern was not observed among PCOS patients undergoing metformin therapy. CONCLUSION: Decreased serum levels of sFas and luteinized granulosa cell DNA fragmentation is observed in patients with PCOS undergoing IVF cycles. Metformin therapy preceding IVF demonstrates an antiapoptotic effect with increased serum levels of sFas, decreased follicular fluid levels of sFasL and prevention of luteinized granulosa cell DNA fragmentation.


Serum Glycan Signatures of Gastric Cancer
Özcan Kabasakal, Süreyya; Ruhaak, L. Renee; Torres, Javier; Cooke, Cara L.; An, Hyun Joo; Hua, Serenus; Williams, Cynthia C.; Dimapasoc, Lauren M.; Kim, Jae Han; Camorlinga-Ponce, Margarita; Rocke, David; Lebrilla, Carlito B.; Solnick, Jay V. (American Association for Cancer Research (AACR), 2014-02-01)
Glycomics, a comprehensive study of glycans expressed in biologic systems, is emerging as a simple yet highly sensitive diagnostic tool for disease onset and progression. This study aimed to use glycomics to investigate glycan markers that would differentiate patients with gastric cancer from those with nonatrophic gastritis. Patients with duodenal ulcer were also included because they are thought to represent a biologically different response to infection with Helicobacter pylori, a bacterial infection tha...
Concentration dependent different action of progesterone on the order, dynamics and hydration states of the head group of dipalmitoyl-phosphatidylcholine membrane
Korkmaz, Filiz; Kirbiyik, Halil; Severcan, Feride (Hindawi Limited, 2005)
Interactions of progesterone with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of progesterone concentration at selected temperatures monitoring both the gel and liquid crystalline phase, by using Fourier Transform Infrared spectroscopy (FTIR). It has been show that the effect of progesterone on membrane dynamics is dependent on progesterone concentration. At 1 mol%, which is close to physiological level, progesterone behaves differently....
The serum angiotensin converting enzyme and lysozyme levels in patients with ocular involvement of ankylosing spondylitis, behcet disease, sarcoidosis and latent tuberculosis
Gurses, Ozlem; Karaismailoglu, Eda (2013-06-01)
Purpose: To evaluate the significance of differences in serum angiotensin converting enzyme and lysozyme levels between the patient groups. Methods: This is a prospective study involving 37 patients with HLAB27+ ankylosing spondylitis, 47 patients with HLAB51+ behcet disease, 29 patients with sarcoidosis and 27 patients with quantiferon+ latent tuberculosis. The patients are included in the study according to the tenets of Decleration of Helsinki. The serum levels of angiotensin converting enzyme and lysoz...
Differential immune activation following encapsulation of immunostimulatory CpG oligodeoxynucleotide in nanoliposomes.
Erikçi, E; Gürsel, Mayda; Gürsel, I (2011-02-01)
The immunogenicity of a vaccine formulation is closely related to the effective internalization by the innate immune cells that provide prolonged and simultaneous delivery of antigen and adjuvant to relevant antigen presenting cells. Endosome associated TLR9 recognizes microbial unmethylated CpG DNA. Clinical applications of TLR9 ligands are significantly hampered due to their pre-mature in vivo digestion and rapid clearance. Liposome encapsulation is a powerful tool to increase in vivo stability as well as...
Genetic aspects of human male infertility: the frequency of chromosomal abnormalities and Y chromosome microdeletions in severe male factor infertility
Vicdan, A; Vicdan, K; Gunalp, S; Kence, Aykut; Akarsu, C; Isik, AZ; Sozen, E (Elsevier BV, 2004-11-10)
Objective: The main purpose of this, study is to detect the frequency and type of both chromosomal abnormalities and Y chromosome microdeletions in patients with severe male factor infertility and fertile control subjects. The association between the genetic abnormality and clinical parameters was also evaluated. Methods: This study was carried out in 208 infertile and 20 fertile men. Results of 208 patients, 1 19 had non-obstructive azoospermia and 89 had severe oligoasthenoteratozoospermia (OAT). Seventee...
Citation Formats
G. Onalan et al., “Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF,” HUMAN REPRODUCTION, pp. 2391–2395, 2005, Accessed: 00, 2020. [Online]. Available: