Date

2018-10-10

Author

Özcengiz, Gülay
Ünsaldi, E.
Aktaş, C.
Ertekin, Ozan
Özcan, S.
Kurt Kızıldoğan, Aslıhan

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In our earlier study, we undertook targeted hom disruption in Streptomyces clavuligerus to improve cephamycin C (CC) yields. In this way, all the carbon coming from aspartate would be directed to l-lysine rather than being shared between the two branches, and secondly, concerted feedback inhibition of aspartokinase would be relieved. This rationale manipulation resulted in up to 4.3-fold and 2-fold increase in intracellular free l-lysine concentration and specific cephamycin C (CC) production, respectively, during stationary phase in chemically defined medium supplemented with l-methionine and l-threonine [1]. There appeared some other consequences of this mutation on cellular physiology since the mutant displayed some developmental alterations as well as red pigmentation. The present study thus focused on global determination of the impact of hom inactivation on primary and secondary metabolic pathway fluxes as well as other physiological functions of our mutant. For this aim, comparative proteomics via LC–MS/MS was taken as an approach followed by quantitative transcript analysis of the selected genes. The study identified 68 differentially expressed proteins, 28 being overrepresented and 40 underrepresented. Apart from many hypothetical/unknown proteins, the identified proteins mainly belonged to the functional classes namely secondary metabolism, amino acid and lipid metabolism, and DNA replication, repair and transcription [2]. Subsequent Q PCR results clearly indicated that protein levels accord well with the transcript levels for the majority of the genes analyzed.

URI

https://hdl.handle.net/11511/32781

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Department of Biology, Article