H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway.

Download

index.pdf

Date

2011-09-01

Author

Terzi Çizmecioğlu, Nihal
Vasiljeva, L
Weissman, J
Buratowski, S

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

116
views

0
downloads

In Saccharomyces cerevisiae, the Nrd1-Nab3-Sen1 pathway mediates the termination of snoRNAs and cryptic unstable transcripts (CUTs). Both Nrd1 and the Set1 histone H3K4 methyltransferase complex interact with RNA polymerase II (Pol II) during early elongation, leading us to test whether these two processes are functionally linked. The deletion of SET1 exacerbates the growth rate and termination defects of nrd1 mutants. Set1 is important for the appropriate recruitment of Nrd1. Additionally, Set1 modulates histone acetylation levels in the promoter-proximal region via the Rpd3L deacetylase and NuA3 acetyltransferase complexes, both of which contain PHD finger proteins that bind methylated H3K4. Increased levels of histone acetylation reduce the efficiency of Nrd1-dependent termination. We speculate that Set1 promotes proper early termination by the Nrd1-Nab3-Sen1 complex by affecting the kinetics of Pol II transcription in early elongation.

Subject Keywords

Cell Biology, Molecular Biology

URI

https://hdl.handle.net/11511/34496

Journal

Molecular and cellular biology

DOI

https://doi.org/10.1128/mcb.05590-11

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin. Vasiljeva, L; Kim, M; Terzi Çizmecioğlu, Nihal; Soares, LM; Buratowski, S (Elsevier BV, 2008-02-15) Within the heterochromatin of budding yeast, RNA polymerase II (RNAPII) transcription is repressed by the Sir2 deacetylase. Although heterochromatic silencing is generally thought to be due to limited accessibility of the underlying DNA, there are several reports of RNAPII and basal transcription factors within silenced regions. Analysis of the rDNA array revealed cryptic RNAPII transcription within the "non-transcribed" spacer region. These transcripts are terminated by the Nrd1 /Sen1 complex and degraded ...
RanGTP induces an effector gradient of XCTK2 and importin alpha/beta for spindle microtubule cross-linking Ems-McClung, Stephanie C.; Emch, Mackenzie; Zhang, Stephanie; Mahnoor, Serena; Weaver, Lesley N.; Walczak, Claire E. (Rockefeller University Press, 2020-02-03) High RanGTP around chromatin is important for governing spindle assembly during meiosis and mitosis by releasing the inhibitory effects of importin alpha/beta Here we examine how the Ran gradient regulates Kinesin-14 function to control spindle organization. We show that Xenopus Kinesin-14, XCTK2, and importin alpha/beta form an effector gradient that is highest at the poles and diminishes toward the chromatin, which is opposite the RanGTP gradient. Importin alpha and beta preferentially inhibit XCTK2 antip...
Embryonic stem cell-derived motoneurons provide a highly sensitive cell culture model for botulinum neurotoxin studies, with implications for high-throughput drug discovery. Kiriş, Erkan; Burnett, JC; Kota, KP; Koh, DC; Wanner, LM; Torres-Melendez, E; Gussio, R; Tessarollo, L; Bavari, S (Elsevier BV, 2011-05-01) Botulinum neurotoxins (BoNTs) inhibit cholinergic synaptic transmission by specifically cleaving proteins that are crucial for neurotransmitter exocytosis. Due to the lethality of these toxins, there are elevated concerns regarding their possible use as bioterrorism agents. Moreover, their widespread use for cosmetic purposes, and as medical treatments, has increased the potential risk of accidental overdosing and environmental exposure. Hence, there is an urgent need to develop novel modalities to counter ...
The effect of valine substitution for glycine in the dimer interface of citrate synthase from Thermoplasma acidophilum on stability and activity Kocabıyık, Semra (Elsevier BV, 2000-08-28) To determine the role of hydrophobic interactions in the dimer interface of citrate synthase (CS) from Thermoplasma (Tp) acidophilum in thermostabilization, we have used site-directed mutagenesis to replace Gly 196 by Val on the helix L of the subunit interface. Recombinant wild-type and Gly 196 mutant TpCS enzymes were largely identical in terms of substrate specificities (K-m for oxaloacetate and acetyl CoA). However, the mutation not only reduced catalytic activity (about 10-fold) (i.e., V-max, K-cat and...
Plasmacytoid Dendritic Cell Response to CpG ODN Correlates with CXCL16 Expression and Is Inhibited by ox-LDL Gürsel, Mayda; GÜRSEL, İHSAN (Hindawi Limited, 2013-01-01) Structurally distinct classes of synthetic CpG oligonucleotides (ODN) differentially activate human immune cells. K-type ODN trigger plasmacytoid dendritic cells (pDCs) to differentiate and produce TNF alpha. In contrast, D-type ODN stimulate large amounts of IFN alpha secretion from pDCs. The cell-surface receptor CXCL16 was previously shown to influence the nature and specificity of CpG ODN-induced immune activation. Here, we evaluated the expression and function of CXCL16 on pDC from healthy volunteers. ...

Citation Formats

N. Terzi Çizmecioğlu, L. Vasiljeva, J. Weissman, and S. Buratowski, “H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway.,” Molecular and cellular biology, pp. 3569–83, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34496.