PRODUCTION OF SUPEROXIDE RADICALS BY SHEEP LIVER NITROFURANTOIN REDUCTASE - IN-VITRO EFFECTS OF NITROFURANTOIN METABOLITES ON DNA

Date

1995-10-01

Author

ISCAN, M
MODARESI, M
Güray, Nülüfer Tülün

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The reduction of nitrofurantoin by purified liver nitrofurantoin reductase was followed by the production of superoxide radicals, which were detected by the reduction of epinephrine. The conditions for the formation of superoxide radicals were optimized. The maximum superoxide radical formation occurred at approximately 3.5 mu g purified reductase, with optimum pH of 7.8 and at 0.05 mM nitrofurantoin concentration. The K-m and V-max values were calculated as 1.95 x 10(-2) mM and 4.81 nmol superoxide formed per minute. An obliteration of the banding pattern was observed on the agarose gel electrophoresis of sheep liver DNA, which was incubated in the nitrofurantoin-nitrofurantoin reductase system, as a possible consequence of the generation of superoxide radicals during the reduction of nitrofurantoin by nitrofurantoin reductase.

Subject Keywords

Cytochrome-C Reductase, Lung, Nitroreductase, Injury

URI

https://hdl.handle.net/11511/36398

Journal

TOXICOLOGY IN VITRO

DOI

https://doi.org/10.1016/0887-2333(95)00016-2

Collections

Department of Biology, Article

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Citation Formats

M. ISCAN, M. MODARESI, and N. T. Güray, “PRODUCTION OF SUPEROXIDE RADICALS BY SHEEP LIVER NITROFURANTOIN REDUCTASE - IN-VITRO EFFECTS OF NITROFURANTOIN METABOLITES ON DNA,” TOXICOLOGY IN VITRO, pp. 743–742, 1995, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36398.