Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Paclitaxel suppresses Tau-mediated microtubule bundling in a concentration-dependent manner.
Date
2017-01-01
Author
Choi, MC
Chung, PJ
Song, C
Miller, HP
Kiriş, Erkan
Li, Y
Wilson, L
Feinstein, SC
Safinya, CR
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
71
views
0
downloads
Cite This
Background: Microtubules (MTs) are protein nanotubes comprised of straight protofilaments (PFs), head to tail assemblies of alpha beta-tubulin heterodimers. Previously, it was shown that Tau, a microtubule-associated protein (MAP) localized to neuronal axons, regulates the average number of PFs in microtubules with increasing inner radius <R-in(MT)> observed for increasing Tau/tubulin-dimer molar ratio Phi(Tau) at paclitaxel/tubulin-dimer molar ratio Lambda(ptxl) = 1/1. Methods: We report a synchrotron SAXS and TEM study of the phase behavior of microtubules as a function of varying concentrations of paclitaxel (1/32 <= Lambda(ptxl) <= 1/4) and Tau (human isoform 3RS, 0 <= Phi(3Rs) <= 1/2) at room temperature. Results: Tau and paclitaxel have opposing regulatory effects on microtubule bundling architectures and microtubule diameter. Surprisingly and in contrast to previous results at Air = 1/1 where microtubule bundles are absent, in the lower paclitaxel concentration regime (Lambda(ptxl) <= 1/4), we observe both microtubule doublets and triplets with increasing Tau. Furthermore, increasing paditaxel concentration (up to Lambda(ptxl) = 1/1) slightly decreased the average microtubule diameter (by similar to 1 PF) while increasing Tau concentration (up to Phi(3RS) = 1/2) significantly increased the diameter (by similar to 2-3 PFs). Conclusions: The suppression of Tau-mediated microtubule bundling with increasing paclitaxel is consistent with paditaxel seeding more, but shorter, microtubules by rapidly exhausting tubulin available for polymerization. Microtubule bundles require the aggregate Tau-Tau attractions along the microtubule length to overcome individual microtubule thermal energies disrupting bundles. General significance: Investigating MAP-mediated interactions between microtubules (as it relates to in vivo behavior) requires the elimination or minimization of paclitaxel. (C) 2016 Elsevier B.V. All rights reserved.
Subject Keywords
Small-angle X-ray scattering
,
Microtubule
,
Paclitaxel
,
Tau
URI
https://hdl.handle.net/11511/36954
Journal
Biochimica et biophysica acta. General subjects
DOI
https://doi.org/10.1016/j.bbagen.2016.09.011
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
Structural properties of an engineered outer membrane protein G mutant, OmpG-16SL, investigated with infrared spectroscopy
Yilmaz, Irem; Yildiz, Ozkan; KORKMAZ ÖZKAN, FİLİZ (Informa UK Limited, 2019-05-31)
The structural and functional differences between wild type (WT) outer membrane protein G and its two mutants are investigated with Fourier transform infrared spectroscopy. Both mutants have a long extension to the primary sequence to increase the number of beta-strands from 14 (wild type) to 16 in an attempt to enlarge the pore diameter. The comparison among proteins is made in terms of pH-dependent conformational changes and thermal stability. Results show that all proteins respond to pH change but at dif...
Abiotic stress tolerance and growth responses of transgenic potato (Solanum tuberosum L. cv. Kennebec) plants expressing rice Osmyb4 gene
AYDIN, GÜLSÜM; Yucel, Meral; Öktem, Hüseyin Avni (2012-09-23)
MYB transcription factors are involved in diverse biochemical and physiological processes such as regulation of secondary metabolism, meristem formation, cell morphogenesis and floral and seed development. They are also involved in certain defence and stress responses and in hormone signalling. In the present study, we developed transgenic potato (Solanum tuberosum L. cv. Kennebec) expressing Oryza sativa myb4 gene, encoding MYB4 transcription factor, driven by either CaMV35S constitutive promoter or cold i...
Gli2 and Gli3 localize to cilia and require the intra-flagellar transport protein polaris for processing and function
Haycraft, CJ; Banizs, B; Aydın Son, Yeşim; Zhang, QH; Michaud, EJ; Yoder, BK (2005-10-01)
Intraflagellar transport (IFT) proteins are essential for cilia assembly and have recently been associated with a number of developmental processes, such as left-right axis specification and limb and neural tube patterning. Genetic studies indicate that IFT proteins are required for Sonic hedgehog (Shh) signaling downstream of the Smoothened and Patched membrane proteins but upstream of the Glioma (Gli) transcription factors. However, the role that IFT proteins play in transduction of Shh signaling and the ...
Inference of large-scale networks via statistical approaches
Ayyıldız Demirci, Ezgi; Purutçuoğlu Gazi, Vilda; Department of Statistics (2019)
In system biology, the interactions between components such as genes, proteins, can be represented by a network. To understand the molecular mechanism of complex biological systems, construction of their networks plays a crucial role. However, estimation of these networks is a challenging problem because of their high dimensional and sparse structures. The Gaussian graphical model (GGM) is widely used approach to construct the undirected networks. GGM define the interactions between species by using the con...
Fatigue behavior of Ti-6Al-4V foams processed by magnesium space holder technique
Asik, E. Erkan; Bor, Sakir (2015-01-05)
Porous Ti-6Al-4V alloys are widely used in the biomedical applications for hard tissue implantation due to their elastic moduli being close to that of bone. In this study, porous Ti-6Al-4V alloys were produced with a powder metallurgical process, space holder technique, where magnesium powders were utilized to generate porosity in the range of 51-65 vol%. The production of porous Ti-6Al-4V alloys was composed of three steps. Firstly, spherical Ti-6Al-4V powders with an average size of 55 mu m were mixed wit...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. Choi et al., “Paclitaxel suppresses Tau-mediated microtubule bundling in a concentration-dependent manner.,”
Biochimica et biophysica acta. General subjects
, pp. 3456–3463, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36954.